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Fumonisin

General information on fumonisin

Fumonisins are carcinogenic, neuro, hepato and pneumo toxic metabolites of Fusarium moniliforme, a mould fungi which grows hostspecific on corn. The dose of fumonisin for the release of toxic effects differs significantly depending on the animal species. A concentration of approx. 5 – 10 mg/kg fumonisin in feed induces neuro toxic effects in horses. In pigs the ingestion of 4 – 16 mg/kg body weight may result in liver cirrhosis and more than 16 mg/kg body weight may lead to pulmonary edema. Chickens tolerate higher concentrations of fumonisin in feed, up to 75 mg/kg. Cattle seem to be insensitive to high fumonisin concentrations.

Fumonisin testsystems from R-Biopharm

We offer different analytical platforms for the analysis of fumonisins as well as standards and reference materials.

FUMONIPREP®

Intended use:

Immunoaffinity columns are used in conjunction with an HPLC system for detection of fumonisin B1, B2 and B3 in a wide range of commodities.

General Information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.

Specifications
Art. No. P31
Test Format 25 wide format immunoaffinity columns (P31).
Sample Preparation The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released by the antibody following elution with solvent. The eluate is collected and derivatised prior to analysis by HPLC.
Detection Limit LOD: B1: 5 ng/ml.
B2: 10 ng/ml.
Cross Reactivity Fumonisin B1, B2 and B3.
Shelf life Shelf life of 18 months at 2 – 8 °C (or 12 months at 21 – 25 °C).
Sensitivity Low % CV, excellent recoveries, capacity and limit of detection exceeds EU Legislation and CEN / AOAC requirements.
Rapid Clean-up in 20 minutes prior to detection by HPLC.
Robust Can be stored at room temperature.
Reliable Uses a monoclonal antibody to selectively isolate and concentrate fumonisin B1, B2 and B3.
Adaptable The columns are suitable for testing a wide range of commodities.
Extraction solvents Methanol and Acetonitrile.
Email Link biotica@otenet.gr

PuriTox Fumonisin

Intended use:

Solid phase clean-up columns for the purification of fumonisins B1, B2 and B3 from cereals.

General Information:

PuriTox Fumonisin columns can be used for simultaneous detection of Fumonisins B1, B2 and B3 by HPLC. The columns reduce background interference therefore producing better chromatography and improving accuracy of results.

Specifications
Art. No. TC-F120
Test Format 25 solid phase gravity flow clean-up columns
Sample Preparation The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.The clean-up columns can be used in conjunction with HPLC for the analysis of pigmented samples.
Detection Limit 100 ng/ml
Cross Reactivity Fumonisin B1, B2 and B3
Shelf life 3 years at 21 – 25 °C.
Rapid Clean-up in 30 minutes prior to detection by HPLC.
Robust Can be stored at room temperature prior to detection by HPLC.
Reliable An effective clean-up system producing cleaner chromatography and increased accuracy.
Adaptable The columns are suitable for testing a wide range of cereal samples.
Extraction solvents Acetonitrile and Methanol.
Email Link biotica@otenet.gr

RIDASCREEN® Fumonisin

Intended use:

RIDASCREEN® Fumonisin is a competitive enzyme immunoassay for the quantitative analysis of fumonisin residues in corn and corn products.

General Information:

Fumonisins are carcinogenic, highly toxic metabolites of Fusarium moniliforme, a mould fungi which grows hostspecific on corn. The toxic concentrations of fumonisin differ significantly depending on the animal species. A concentration of approx. 5 – 10 mg/kg fumonisin in feed induces neurotoxic effects in horses. In pigs the ingestion of 4 – 16 mg/kg body weight may result in liver cirrhosis and more than 16 mg/kg body weight may lead to pulmonary edema. Chickens tolerate higher concentrations of fumonisin in feed, up to 75 mg/kg. Cattle seem to be insensitive to high fumonisin concentrations.

Specifications
Art. No. R3401
Test Format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Standard Range 0 ppm (zero standard), 0.025 ppm, 0.074 ppm, 0.222 ppm, 0.666 ppm, 2 ppm
Sample Preparation extraction, filtration and dilution
Incubation Time 45 min
Detection Limit approx. 25 µg/kg (ppb)(corresponding to the standard substance)
Cross Reactivity Fumonisin B1: 100 %
Fumonisin B2: approx. 40 %
Fumonisin B3: approx. 100 %

RIDASCREEN®FAST Fumonisin

Intended use:

RIDASCREEN®FAST Fumonisin is a competitive enzyme immunoassay for the quantitative analysis of fumonisin in cereals and feed.
The test is approved by the Grain Inspection, Packers and Stockyards Administration of the United States Department of Agriculture (USDA/GIPSA) for corn.

CERTIFICATE NO. FGIS 2012 – 030

General Information:

Fumonisins are carcinogenic, highly toxic metabolites of Fusarium moniliforme, a mould fungi, which grows hostspecific on corn. The toxic concentrations of fumonisin differ significantly depending on the animal species. A concentration of approx. 5 – 10 mg/kg fumonisin in feed induces neurotoxic effects in horses. In pigs the ingestion of 4 – 16 mg/kg body weight may result in liver cirrhosis and more than 16 mg/kg bw. may lead to pulmonary edema. Chickens tolerate higher concentrations of fumonisin in feed, up to 75 mg/kg. Cattle seem to be insensitive to high fumonisin concentrations.

Specifications
Art. No. R5602
Test Format Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range 0 ppm (zero standard), 0.222 ppm, 0.666 ppm, 2 ppm, 6 ppm
Sample Preparation extraction, filtration, dilution
Incubation Time 15 min
Detection Limit 0.222 mg/kg (ppm)(corresponding to the standard substance)

RIDA®QUICK Fumonisin

Intended use:

RIDA®QUICK Fumonisin is a semi-quantitative immunochromatographic test in strip formate for the detection of fumonisin in corn.

General Information:

Fumonisins are carcinogenic, highly toxic metabolites of Fusarium moniliforme, which grows hostspecific on corn. The toxic concentrations of Fumonisin differ significantly depending on the animal species.

Specifications
Art. No. R5604
Test Format 20 x test strips (one for each determination, separately packed)
Sample Preparation homogenization and extraction
Incubation Time 5 min
Detection Limit approx. 0.8 mg/kg (ppm) or approx. 4.0 mg/kg (ppm) depending on the extraction
A positive result indicates that the sample has a fumonisin content of ≥ 0.8 mg/kg (ppm) or ≥ 4.0 mg/kg (ppm).
Specificity The RIDA®QUICK Fumonisin test detects fumonisin in corn samples.
The test has been validated for this matrix.

RIDA®QUICK Fumonisin RQS

Intended use:

RIDA®QUICK Fumonisin RQS is a quantitative (evaluation with RIDA®QUICK SCAN) immunochromatographic test in strip format for the detection of fumonisin in corn.

General Information:

Fumonisins are carcinogenic, highly toxic metabolites of Fusarium moniliforme, which grows hostspecific on corn. The toxic concentrations of fumonisin differ significantly depending on the animal species.

Specifications
Art. No. R5606
Test Format 20 x test strips (one for each determination, separately packed)
Sample Preparation homogenization and extraction
Incubation Time 5 min
Detection Limit approx. 0.8 mg/kg (ppm) or approx. 4.0 mg/kg (ppm) – depending on the extraction
Specificity The RIDA®QUICK Fumonisin test detects fumonisin in corn samples.
The test has been validated for this matrix.

Mycotoxins

Mycotoxin Standards
Aflatoxin
Citrinin
DON
Fumonisin
Multi-Toxins
Ochratoxin
Patulin
Reference Materials
T-2 Toxin / HT-2 toxin
Trichothecenes
Zearalenone
Accessories