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Ochratoxin

General information on ochratoxin

The mycotoxin ochratoxin A is formed by fungi of the species Aspergillus and Penicillium. Apart from a marked nephrotoxicity, ochratoxin A displays hepatotoxic, teratogenic, carcinogenic and immunosuppressive properties. There is a risk to human health not only through the intake of contaminated foods of vegetable origin, but also through foods of animal origin. Ochratoxin A was
detected in pig blood and kidneys, as well as in human blood and mother’s milk.

Ochratoxin testsystems from R-Biopharm

We offer different analytical platforms for the analysis of ochratoxin as well as standards and reference materials.

OCHRACARD

Intended use:

A qualitative screening card for detection of ochratoxin A at various levels in food and feed commodities.

General Information:

The kit is based on monoclonal antibody technology which has the advantage of being highly specific and sensitive while the test format is rapid and simple to perform. The screening procedure is intended to serve as an indicator of the presence of toxins at various screening levels according to European and International legislation.

Specifications
Art. No. P48
Test Format 10 cards containing 20 tests and controls.
Sample Preparation The toxin is extracted from the sample, filtered and passed through an immunoaffinity column before being diluted and applied to the card. The conjugate is applied to the membrane and unbound conjugate is then removed by washing. A colourless substrate is added and the card is incubated for five minutes. Finally a stop solution is applied to the membrane. A purple spot must appear at the control site to indicate that the test is valid. A purple spot at the sample site shows that the contamination is less that the cut off value of the card. No colour at the sample site indicates contamination at a higher level than the cut off of the card.
Incubation Time 5 minutes.
Detection Limit Varies depending on dilution used.
Shelf life 10 months at 2 – 8 °C.
Sensitivity Complies with current EU Legislation and compares well to ochratoxin A analysis by HPLC.
Rapid The assay time is only 30 minutes.
Robust Cards can be stored in the dark to provide a permanent record of results.
Easy to use The results can be read visually so there is no need to buy expensive detection equipment.
Reliable Uses a monoclonal antibody to detect ochratoxin A.
Adaptable Methods available for a wide range of commodities.
Extraction solvents Methanol.
Email Link biotica@otenet.gr

OCHRATOXIN CLEAN-UP COLUMNS

Intended use:

Solid phase clean-up columns for the purification of ochratoxin from food and feed commodities.

General Information:

OCHRATOXIN CLEAN-UP COLUMNS can be used for the clean-up of pigmented samples before OCHRACARD or ELISA. The solid phase columns reduce background interference and improve the accuracy of results.

 

Specifications
Art. No. P13
Test Format 50 solid phase clean-up columns.
Sample Preparation Extraction and filtration.
Shelf life 2 years at 21 – 25 °C.
Rapid Clean-up in 10 minutes prior to detection by OCHRACARD or ELISA.
Robust Can be stored at room temperature.
Reliable An effective clean-up system producing cleaner filtrates for use in OCHRACARD or with ELISA therefore increasing the accuracy.
Adaptable The columns are suitable for testing a wide range of commodities.
Email Link biotica@otenet.gr

OCHRAPREP®

Intended use:

Immunoaffinity columns for use in conjunction with an HPLC system for detection of ochratoxin A in a wide range of commodities.

General Information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.

 

Specifications
Art. No. P14 / P14B
Test Format 10 wide format immunoaffinity columns (P14).
50 wide format immunoaffinity columns (P14B).
Sample Preparation The columns contain a gel suspension of monoclonal antibody specific to the toxin of interest. Following extraction of the toxin the sample extract is filtered, diluted and passed through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released by the antibody following elution with solvent. The eluate is collected prior to analysis by HPLC.
Detection Limit LOD: 0.05 ng/ml
Cross Reactivity Ochratoxin B (OTB).
Shelf life 18 months at 2 – 8 °C (or 12 months at 21 – 25 °C).
Sensitivity Low % CV, excellent recoveries, capacity and limit of detection exceeds EU Legislation and CEN / AOAC requirements.
Approvals Used in CEN collaborative trials. Methods using these columns have been approved by the AOAC.
Rapid Clean up in 20 minutes prior to detection by HPLC.
Robust Can be stored at room temperature.
Reliable Uses a monoclonal antibody to selectively isolate and concentrate ochratoxin A.
Adaptable The columns are suitable for testing a wide range of commodities.
Extraction solvents Methanol or Acetonitrile.
Email Link biotica@otenet.gr

AFLAOCHRA PREP®

Intended use:

Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxin B1, B2, G1, G2 and ochratoxin A in a wide range of commodities in one HPLC run.

General Information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.

Specifications
Art. No. P89 / P89B
Test Format 10 narrow format immunoaffinity columns (P89).
50 narrow format immunoaffinity columns (P89B).
Sample Preparation The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are released from the antibody following elution with solvent. The eluate is collected and injected prior to analysis by HPLC.
Detection Limit LOD: B1 – 0.031 ng/ml
B2 – 0.015 ng/ml
G1 – 0.015 ng/ml
G2 – 0.007 ng/ml
OTA – 0.05 ng/ml
Cross Reactivity Ochratoxin B (OTB)
Shelf life 18 months at 2 – 8 °C (or 12 months at 21 – 25 °C).
Sensitivity Low % CV, excellent recoveries, capacity and limit of detection exceeds EU Legislation and CEN / AOAC requirements.
Rapid Only one extraction and a single column clean up required taking as little as 20 minutes prior to detection by HPLC. Simultaneous detection of total aflatoxins and ochratoxin A in one HPLC run.
Robust Can be stored at room temperature.
Reliable Uses monoclonal antibodies to selectively isolate and concentrate aflatoxins B1, B2, G1, G2 and ochratoxin A.
Adaptable The columns are suitable for testing a wide range of commodities.
Extraction solvents Methanol.
Additional Information Cost Effective: No need for two separate analyses, saving time and money.
Email Link biotica@otenet.gr

RIDA® Ochratoxin A column

Intended use:

RIDA® Ochratoxin A column (Art. No.: R1303) are immunoaffinity columns for sample clean up prior to analysis of Ochratoxin A in food and feed.

General Information:

RIDA® Ochratoxin A column are usable in combination with the enzyme immunoassays (R1311, RIDASCREEN® Ochratoxin A 30/15 and R5402, RIDASCREEN®FAST Ochratoxin A) for the quantitative determination of Ochratoxin A.
The sample preparation with the immunoaffinity columns simplifies and enhances the sample clean up procedure. Pure extracts are obtained, which can be analyzed by different analytical methods.

Specifications
Art. No. R1303
Test Format 10 immunoaffinity columns
Detection Limit depending on sample volume applied and on detection method

RIDASCREEN® Ochratoxin A 30/15

Intended use:

RIDASCREEN® Ochratoxin A 30/15 test is a competitive enzyme immunoassay for the quantitative analysis of ochratoxin A in cereals, feed, beer and pig serum.

General Information:

The mycotoxin ochratoxin A is formed by fungi of the species Aspergillus and Penicillium. Apart from a marked nephrotoxicity, ochratoxin A displays hepatotoxic, teratogenic, carcinogenic and immunosuppressive properties.
There is a risk to human health not only through the intake of contaminated foods of vegetable origin, but also through foods of animal origin. Ochratoxin A was detected in pig blood and kidneys, as well as in human blood and mother’s milk.

Specifications
Art. No. R1311
Test Format Microtiter plate with 96 wells (12 strips with 8 wells each)
Standard Range 0 ppt (zero standard), 50 ppt, 100 ppt, 300 ppt, 900 ppt, 1800 ppt
Sample Preparation cereals and feed: extraction, centrifugation or filtration
beer and pig serum: extraction, centrifugation, filtration, shaking, second extraction, centrifugation and evaporation
Incubation Time 45 min
Detection Limit cereals and feed: 2.5 ppb (sample preparation for a rapid and easy screening)
cereals and feed: 1.25 ppb (Sample preparation for a more sensitiv screening)
beer and pig serum: approx. 50 ppt
Cross Reactivity Ochratoxin A: 100 %
Ochratoxin C: 44 %
Ochratoxin B: 14 %
Ochratoxin α: < 0.1 %

RIDASCREEN®FAST Ochratoxin A

Intended use:

RIDASCREEN®FAST Ochratoxin A îs a competitive enzyme immunoassay for the quantitative analysis of ochratoxin A in cereals and feed.

General Information:

The mycotoxin ochratoxin A is formed by fungi of the species Aspergillus and Penicillium. Apart from a marked nephrotoxicity, ochratoxin A displays hepatotoxic, teratongenic, carcinogenic and immunsuppressive properties.
There is a risk to human health not only through the intake of contaminated foods of vegetable origin, but also through foods of animal origin. Ochratoxin A was detected in pig blood and kidneys, as well as in human blood and mother’s milk.

Specifications
Art. No. R5402
Test Format Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range 0 ppb (zero standard), 5 ppb, 10 ppb, 20 ppb, 40 ppb
Sample Preparation extraction, filtration and dilution
Incubation Time 15 min
Detection Limit 5 µg/kg (ppb)

Mycotoxins

Mycotoxin Standards
Aflatoxin
Citrinin
DON
Fumonisin
Multi-Toxins
Ochratoxin
Patulin
Reference Materials
T-2 Toxin / HT-2 toxin
Trichothecenes
Zearalenone
Accessories