START TYPING AND PRESS ENTER TO SEARCH

Zearalenone

General information on zearalenone

The mycotoxin zearalenone is formed by fungi of the genus Fusarium. Zearalenone is a phytohormone which displays, apart from its anabolic properties, mainly estrogenic effects. Because of its estrogenic properties, zearalenone may induce fertility disorders in animals with clinical signs of hyperestrogenism – an aspect of a disease which although reported mainly in hogs, is described in other species such as cow, horse and sheep, too.
The potential health risk for man induced by this mycotoxin, which is taken up with foods of vegetable or animal origin, is  extensively discussed.

Zearalenone testsystems from R-Biopharm

We offer different analytical platforms for the analysis of zearalenone as well as standards and reference materials.

DZT MS-PREP®

Intended use:

Multi-mycotoxin immunoaffinity columns for simultaneous detection of deoxynivalenol, zearalenone, T-2 and HT-2 in a wide range of commodities in one LC-MS/MS run.

General Information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.

Specifications
Art. No. P73 / P73B
Test Format 10 narrow format immunoaffinity columns (P73).
50 narrow format immunoaffinity columns (P73B).
Sample Preparation The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released by the antibody following elution with solvent. The eluate is collected prior to analysis by LC-MS/MS.
Detection Limit LOD: DON – 0.1 ng/ml
ZON – 0.05 ng/ml
T-2 – 0.05 ng/ml
HT-2 0.05 ng/ml
Cross Reactivity 3-acetyl DON, α-Zearalenol (α-ZOL), ß-Zearalenol (ß-ZOL), Zearalanone (ZAN), α-Zearalanol (α-ZAL) and Zeranol.
Shelf life 18 months at 2 – 8 °C (or 12 months at 21 – 25 °C).
Sensitivity Low % CV, excellent recoveries and limit of detection complies with EU Legislation.
Rapid Only one extraction and a single column clean-up required taking as little as 20 minutes prior to detection by LC-MS/MS. Simultaneous detection of deoxynivalenol, zearalenone, T-2 and HT-2 in one LC-MS/MS run.
Robust Can be stored at room temperature.
Reliable Uses monoclonal antibodies to selectively isolate and concentrate deoxynivalenol, zearalenone, T-2 and HT-2.
Adaptable The columns are suitable for testing a wide range of commodities.
Extraction solvents Methanol and Acetonitrile.
Additional Information Cost Effective: No need for three separate analyses, saving time and money.Improved Clean-Up: Reduces interference and therefore the need to use matrix matched standards.
Email Link biotica@otenet.gr

EASI-EXTRACT® ZEARALENONE

Intended use:

Immunoaffinity columns for use in conjunction with an HPLC system for detection of zearalenone in a wide range of commodities.

General Information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.

Specifications
Art. No. RP91 / RP90
Test Format 10 wide format immunoaffinity columns (RP91).
50 wide format immunoaffinity columns (RP90).
Sample Preparation The columns contain a gel suspension of monoclonal antibody specific to the toxin of interest. Following extraction of the toxin the sample extract is filtered, diluted and passed through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released by the antibody following elution with solvent. The eluate is collected prior to analysis by HPLC.
Detection Limit 1.56 ng/ml.
Cross Reactivity α-Zearalenol (α-ZOL), ß-Zearalenol (ß-ZOL), Zearalanone (ZAN), α-Zearalanol (α-ZAL) and Zeranol.
Shelf life 18 months at 2 – 8 °C (or 12 months at 21 – 25 °C).
Sensitivity Low % CV, excellent recoveries, capacity and limit of detection exceeds EU Legislation and CEN requirements.
Approvals Used in CEN and BCR collaborative trials.
Rapid Clean up in 20 minutes prior to detection by HPLC.
Robust Can be stored at room temperature.
Reliable Uses a monoclonal antibody to selectively isolate and concentrate zearalenone.
Adaptable The columns are suitable for testing a wide range of commodities.
Extraction solvents Methanol or Acetonitrile.
Additional Information Zearalenone standards are available from R-Biopharm.
Email Link biotica@otenet.gr

PuriTox MultiToxin

Intended use:

Solid phase clean-up columns for the purification of aflatoxins and zearalenone from cereals.

General Information:

PuriTox MultiToxin columns can be used for the simultaneous detection of aflatoxins and zearalenone by HPLC or GC. The columns reduce background interference therefore producing better chromatography and improving accuracy of results.

Specifications
Art. No. TC-M160
Test Format 25 solid phase syringe format clean-up columns
Sample Preparation The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.The clean-up columns can be used in conjunction with HPLC or GC for the analysis of pigmented samples.
Detection Limit Aflatoxin – 1 ng/ml
Zearalenone – 50 ng/ml
Shelf life 3 years at 21 – 25 °C
Rapid Clean-up in 30 minutes prior to detection by HPLC or GC.
Robust Can be stored at room temperature.
Reliable An effective clean-up system producing cleaner chromatography and increased accuracy.
Adaptable The columns are suitable for testing a wide range of cereal samples.
Extraction solvents Acetonitrile and Methanol.
Email Link biotica@otenet.gr

RIDASCREEN® Zearalenon

Intended use:

RIDASCREEN® Zearalenon is a competitive enzyme immunoassay for the quantitative analysis of zearalenone residues in cereals, feed, beer, serum and urine.

General Information:

The mycotoxin zearalenone is formed by fungi of the genus Fusarium. Zearalenone is a phytohormone which displays, apart from its anabolic properties, mainly estrogenic effects. Because of its estrogenic properties, zearalenone may induce fertility disorders in animals with clinical signs of hyperestrogenism – an aspect of a disease which although reported mainly in hogs, is described in other species such as cow, horse and sheep, too.
The potential health risk for man induced by this mycotoxin, which is taken up with foods of vegetable or animal origin, is extensively discussed.

Specifications
Art. No. R1401
Test Format Microtiter plate with 96 wells (12 strips with 8 wells each)
Standard Range 0 ppt (zero standard), 50 ppt, 150 ppt, 450 ppt, 1350 ppt, 4050 ppt
Sample Preparation cereals and feed: extraction, filtration and dilution
beer: removing of CO2 and dilution
serum and urine: chromatographic purification by means of RIDA® C18 column (Art. No.: R2002)
Incubation Time 2 h 30 min
Detection Limit cereals and feed: approx. 1750 ppt
beer: approx. 250 ppt
serum and urine: approx. 50 ppt(corresponding to the stanadard substance)
Cross Reactivity The specificity of the RIDASCREEN® Zearalenon test was established by analyzing the cross-reactivity to corresponding mycotoxins in buffer system.Zearalenone: 100 %
α-Zearalenol: approx. 41.6 %
Zeranol (Zearalanol): approx. 27.7 %
β-Zearalenol: approx. 13.8 %

RIDASCREEN®FAST Zearalenon

Intended use:

RIDASCREEN®FAST Zearalenon is a competitive enzyme immunoassay for the quantitative analysis of zearalenone in cereals and feed.

The test was validated with different matrices of cereals and composed feed.

General Information:

The mycotoxin zearalenone is formed by fungi of the genus Fusarium. Zearalenone is a phytohormone which displays, apart from its anabolic properties, mainly estrogenic effects. Because of its estrogenic properties, zearalenone may induce fertility disorders in animals with clinical signs of hyperestrogenism – an aspect of a disease which although reported mainly in hogs, is described in other species such as cows, horses and sheep.
The potential health risk for man induced by this mycotoxin, which can be ingested with foods of vegetable or animal origin, has been documented extensively.

Specifications
Art. No. R5502
Test Format Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range 0 ppb (zero standard), 50 ppb, 100 ppb, 200 ppb, 400 ppb
Sample Preparation extraction, filtration and dilution
Incubation Time 15 min
Detection Limit limit of detection:17 – 41 µg/kg (ppb)limit of quantification: 50 µg/kg (ppb)

RIDASCREEN®FAST Zearalenon SC

Intended use:

RIDASCREEN®FAST Zearalenon SC is a competitive enzyme immunoassay for the quantitative analysis of zearalenone in cereals.

 

General Information:

The mycotoxin zearalenone is formed by fungi of the genus Fusarium. Zearalenone is a phytohormone which displays, apart from its anabolic properties, mainly estrogenic effects. Because of its estrogenic properties, zearalenone may induce fertility disorders in animals with clinical signs of hyperestrogenism – an aspect of a disease which although reported mainly in hogs, is described in other species such as cows, horses and sheep.
The potential health risk for humans induced by this mycotoxin, which can be ingested with foods of vegetable or animal origin, has been discussed extensively.

Specifications
Art. No. R5505
Test Format Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range Only standard 1 (zero standard) is included in the test kit.
For calculation of results with the standard curve the measured absorbance for standard 1 (0 ppb) as well as the B/B0 values for standards 2 – 7 (25, 50, 100, 200, 400, 1000 ppb) provided with the QS-certificate are needed.
Sample Preparation extraction, filtration, dilution
Incubation Time 15 min
Detection Limit Limit of detection: 5 μg/kg (ppb)
Limit of quantification: 16 μg/kg (ppb)(corresponding to the standard substance)

[/toggle] [/tab2] [tab3]

RIDA®QUICK Zearalenon RQS

Intended use:

RIDA®QUICK Zearalenon RQS is a quantitative (evaluation with RIDA®QUICK SCAN) immunochromatographic test in strip format for the detection of zearalenone in corn.

General Information:

The mycotoxin zearalenone is formed by fungi of the genus Fusarium. Zearalenone is a phytohormone which displays, apart from its anabolic properties, mainly estrogenic effects. Because of its estrogenic properties, zearalenone may induce fertility disorders in animals with clinical signs of hyperestrogenism – an aspect of a disease which although reported mainly in hogs, is described in other species such as cow, horse and sheep, too.
The potential health risk for man induced by this mycotoxin, which is taken up with foods of vegetable or animal origin, is extensively discussed.

Specifications
Art. No. R5504
Test Format 20 x test strips (one for each determination, separately packed)
Sample Preparation homogenization and extraction
Incubation Time 5 min
Detection Limit approx. 75 μg/kg (ppb)
Specificity The RIDA®QUICK Zearalenon RQS test detects zearalenone in corn samples.
The test has been validated for this matrix.

Mycotoxins

Mycotoxin Standards
Aflatoxin
Citrinin
DON
Fumonisin
Multi-Toxins
Ochratoxin
Patulin
Reference Materials
T-2 Toxin / HT-2 toxin
Trichothecenes
Zearalenone
Accessories