Soy
RIDA®QUICK Soya
Intended use:
RIDA®QUICK Soya can be used as a swab test for the soya determination on surfaces in the hygiene control and for the qualitative detection of soya in raw material and processed food. The test has been developed for the detection of low amounts of soya (contamination).
For the sample preparation, RIDA QUICK Soya accessory pack (Z7103) is recommended.
For the sample preparation from foods, the Soya extraction (R7113) should be used.
| Art. No. | R7103 |
|---|---|
| Test Format | 25 x dip sticks in reclosable tube (one for each determination), Conjugate, Extraction buffer, 30 Plastic tubes, 25 Tubes 26 Swabs, 50 pipette tips |
| Sample Preparation | homogenization, extraction and centrifugation |
| Incubation Time | 5 min |
| Detection Limit | – on surfaces approx. 0.5 μg soya protein / 100 cm2 – of soya flour in wheat flour approx. 0.5 mg/kg soya protein – in processed food approx. 10 mg/kg soya protein (depending on the matrix) |
RIDASCREEN®FAST Soya
Intended use:
RIDASCREEN®FAST Soya (Art. No. R7102) is a sandwich enzyme immunoassay for the quantitative analysis of native and processed soya proteins in food. Such products are beverages and food as sausages, dressings, bakery products, ice, chocolate, soup, sauce, margarine, etc.
General Information:
Soya proteins can be present as an ingredient or as a contamination in raw and processed products. Several soya proteins can cause allergic reactions. According to the EU Directive 2003/89/EG from November 10th 2003, soya and products thereof must be declared on food labels. Similar regulations exist e.g. in the USA, Canada, Australia and New Zealand.
Soybeans (mature seeds, raw) contain approx. 35 % proteins. Soya proteins have a similar amino acid composition as proteins from animal sources and have a high percentage of essential amino acids. Therefore, soya is one of the few plants which are considered as a valuable source of proteins. Thus, soya is widely used as substitute for proteins from animal sources (e.g. in tofu, soya milk or soya yoghurt). Besides the high protein content, soya beans are also rich in fat (approx. 20 %) and are used for the productions of oils and fats.
The usage of soya as food and feed has strongly increased in the last decades, leading to an increase in allergic reactions as well.
| Art. No. | R7102 |
|---|---|
| Test Format | Microtiter plate with 48 wells(6 strips with 8 removable wells each) |
| Standard Range | 0 ppb (zero standard), 5 ppb, 10 ppb, 20 ppb, 40 ppb, 80 ppb gliadin |
| Sample Preparation | homogenization and extraction |
| Incubation Time | 30 min |
| Detection Limit | 0.24 mg/kg (0.15 – 0.32 depending on the matrix), the kit is suitable for automation |
ELISA- Accessories
Intended use:
The processed assay controls are used to control the extraction, the test implementation and the handling of the RIDASCREEN®FAST Soya (R7102).
Comment:
The Set of 3 processed assay controls is composed of homogenized cookies with a defined content of soya.
The soya assay controls are not a reference material. For validation of the RIDASCREEN®FAST Soya kit, spiked samples should be used.
General Information:
Each assay control contains 2 g homogenized processed cookies
Gliadin Assay Control A (negative): soya-free cookie
Gliadin Assay Control B & C (positives): different concentrations of soya-free and a
soya-containing cookie were mixed.
| Art. No. | R7132 |
|---|---|
RIDA® QUICK Soya accessory pack
Intended use:
The material of the RIDA®QUICK accessory pack (Art. No. Z7103) is recommended to accomplish the swab test with the RIDA®QUICK Soya (Art. No. R7103).
The laboratory equipment of the RIDA®QUICK accessory pack is especially compiled for the eradication of the sample preparation of RIDA®QUICK Soya.
| Art. No. | Z7103 |
|---|---|
| Test Format | Test tube holder, floating rack, pipette |
RIDA®QUICK Soya Extraction buffer
Intended use:
The RIDA®QUICK Soya Extraction buffer is used for the sample preparation for the determination with RIDA®QUICK Soya (Art. No. R7103).
| Art. No. | R7113 |
|---|---|
| Test Format | 2 x 100 ml |
SureFood® ALLERGEN Soya – NEW
Intended use:
The test detects soya DNA according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg soya / kg food sample and a detection system including a standard dilution is required.
For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.
General Information:
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent Mx3005P.
| Art. No. | S3601 |
|---|---|
| Test Format | 100 reactions* |
| Sample Preparation | 1. DNA preparation, e.g. SureFood® PREP ALLERGEN 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run |
| Detection Limit | Depending on the sample matrix, processing grade, DNA preparation and DNA contant, the detection is limited to < 5 DNA copies / ≤ 0.4 ppm. |
| Shelf life | 12 month |
| Additional Information | The detection system system can be used on all available real-time PCR cyclers.ABI Prism Eppendorf realplex iCycler Rotorgene LightCycler |
*Includes Internal Amplification Control (IAC) in VIC/HEX channel. SureFood®QUANTARD Allergen 40 must be used for quantifiction.
SureFood® ALLERGEN 4plex Soya/Celery/Mustard+IAC
Intended use:
Soya, Celery and Mustard detection by real-time PCR in one run.
The multiplex test detects DNA of soya, celery and mustard according to directive (EC) 1169/2011.
For DNA-preparation the use of SureFood® PREP Advanced, protocol 1 is recommended.
General Information:
The real-time PCR assay can be used with established real-time PCR instruments, equipped for the detection of four fluorescence emissions at 510 nm, 580 nm, 610 nm and 660 nm (FAM, VIC/HEX, ROX and Cy5) at the same time. The technical validation was performed on Agilent Mx3005P, Roche LightCycler® 480 II*, Applied Biosystems 7500, BioRad CFX96 und Qiagen Rotor-Gene Q.
| Art. No. | S3401 |
|---|---|
| Test Format | 100 reactions |
| Sample Preparation | 1. DNA preparation, e.g. SureFood® PREP ALLERGEN 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run |
| Detection Limit | Depending on the sample matrix, processing grade, DNA preparation and DNA contant, the detection is limited to < 5 DNA copies / ≤ 0.4 ppm. |
| Shelf life | 12 month |
| Additional Information | The detection system system can be used on all available real-time PCR cyclers.ABI Prism Eppendorf realplex iCycler Rotorgene LightCycler |