Sulfonamides

Sulfonamides (Sulfonamide , Sulfamethazine, Dapsone) are widely used as feed additives, mainly for fattening of calves and pigs. Combined with inhibitors of dihydrofolate reductase such as trimethoprim, tetroxoprim, or pyrimethamine sulfonamides are also used in veterinary medicine for the treatment of intestinal infections, mastitis, pulmonitis and other (systemic) diseases. Sulfonamide residues may therefore occur in food of animal origin such as meat and milk. Particularly the transmission of the carcinogenic sulfamethazine represents a threat to human health. According to the EU law, a maximum residue limit (MRL) for all substances of the sulfonamide-group of 100 µg/kg (ppb) in muscle, fat, liver and kidney and of 100 µg/l (ppb) in milk is valid

RIDASCREEN® Sulfamethazin

Art. No.: R3011

Intended use:

RIDASCREEN® Sulfamethazin is a competitive enzyme immunoassay for the quantitative determination of sulfamethazine in milk, meat, honey, liver, kidney, fish, shrimp and egg.

General information:

Sulfonamides are widely used as feed additives, mainly for fattening of calves and pigs. Combined with inhibitors of dihydrofolate reductase such as trimethoprim, tetroxoprim, or pyrimethamine sulfonamides are also used in veterinary medicine for the treatment of intestinal infections, mastitis, pneumonitis and other (systemic) diseases. Sulfonamide residues may therefore occur in food of animal origin such as meat and milk. Particularly the transmission of the carcinogenic sulfamethazine represents a threat to human health.  The EU established a maximum residue limit (MRL) for all substances of the sulfonamide-group of 100 μg/kg (ppb) in muscle, fat, liver, kidney and milk.

Accessories:

  • RIDA® Sulfamethazin Spiking Solution R3098
Specifications
Art. No. R3011
Test format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Incubation time 45 min

RIDA® Sulfamethazin Spiking Solution

Art. No.: R3098

Intended use:

RIDA® Sulfamethazin Spiking Solution can be used for the production of positive control samples suitable for the validation (e.g. determination of the recovery rate) of the RIDASCREEN® Sulfamethazin test (R3001).

Specifications
Art. No. R3098
Test format Concentration: 10 μg/ml
Volume: 1 ml
Sulfamethazine in buffer, ready to use

RIDASCREEN® Sulfonamide

Art. No.: R3004

Intended use:

RIDASCREEN® Sulfonamide is a competitive enzyme immunoassay for the quantitative analysis of sulfonamides in egg, meat (chicken and pork), fish, shrimp, honey and milk.

General information:

Sulfonamides are widely used as feed additives, mainly for fattening of calves and pigs. Combined with inhibitors of dihydrofolate reductase such as trimethoprim, tetroxoprim, or pyrimethamine sulfonamides are also used in veterinary medicine for the treatment of intestinal infections, mastitis, pulmonitis and other (systemic)  diseases. Therefore sulfonamide residues may occur in food of animal origin such as meat and milk and were found frequently abroad. According to the EU-Law a maximum residue limit for all substances of the sulfonamide-group of 100 µg/kg in muscle, fat, liver and kidney and of 100 µg/l in milk is valid.

Accessories:

  • RIDA® Sulfamethazin Spiking Solution R3099
Specifications
Art. No. R3004
Test format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Sample preparation milk: after 1:5 dilution direct use in the assay
meat (chicken and pork), egg, fish, shrimp: homogenization, extraction, centrifugation, evaporation and degreasing
honey: extraction, RIDA® C18 column purification and dilution
Incubation time 1 h 15 min
Detection limit meat (chicken), egg: 1.5 µg/kg (ppb)
meat (pork), fish, shrimp, honey: 2 µg/kg (ppb)
milk: 3.5 µg/l (ppb)(corresponding to the standard substance)
Cross reactivity Sulfamethoxypyridazine: approx. 100 %
Sulfapyridine: > 100 %
Sulfamethoxydiazine: approx. 75 %
Sulfamethoxazole: approx. 58 %
Sulfadimethoxine: approx. 41 %
Sulfaquinoxaline: approx. 34 %
Sulfachloropyridazine: approx. 19 %
Sulfamerazine: approx. 18 %
Sulfadiazine: approx. 15 %
Sulfamethizole: approx. 14 %
Sulfadoxine: approx. 10 %
Sulfachloropyrazine: approx. 9 %
Sulfaguanidine: approx. 5 %
Sulfaphenazole, Sulfamethazine, Sulfisoxazole, Sulfanilamide: approx.  2 %
Sulfacetamide: approx. 1 %

RIDA® Sulfonamide/Sulfamethoxypyridazin Spiking Solution

Art. No.: R3099

Intended use:

RIDA® Sulfamethoxypyridazin-Spiking Solution can be used for the production of positive control samples suitable for the validation (e.g. determination of the recovery rate) of the RIDASCREEN® Sulfonamide test, R3004.

Specifications
Art. No. R3099
Test format Concentration: 0.1 μg/ml
Volume: 1 ml
Sulfamethoxypyridazine in buffer, ready to use

EuroProxima Dapsone

Art. No.: 5101DAP

Intended use:

A competitive enzyme immunoassay for screening and quantitative analysis of dapsone in various matrices.

General information:

Dapsone is an antibiotic used in animals to prevent and treat diseases such as streptococcal mastitis and coccidiosis of cattle. Dapsone is listed in table 2 of Regulation (EC) no. 37/2010. Veterinary medicinal products in this table are banned for use in/treatment of food-producing animals. A technical guide has been published by the Community Reference Laboratories in which a recommended concentration of 5 ppb has been published for dapsone in muscle, meat and milk.

Specifications
Art. No. 5101DAP
Test format Microtiter plate, 96 wells.
Sample preparation For shrimp, egg and milk fast and efficient methods are included in the kit manual.
Incubation time 150 minutes
Detection limit Shrimp 0.05 ppb
Egg 0.1 ppb
Milk 0.06 ppb
Cross reactivity Dapsone 100%, Sulfaquinoxaline 8.7%