Πουλερικά (Poultry): Κοτόπουλο (Chicken) /Γαλοπούλα (Turkey)
SureFood® ANIMAL ID POULTRY IAAC
Art. No.: S6125
Intended use:
Qualitative detection of poultry DNA.Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).
For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
General information:
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0*, Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Applied Biossystems 7500, Bio-Rad CFX96 Agilent Mx3005P.
*note: A Color Compensation is necessary for users of the Roche LightCyclerR 2.0. The SureCC Color Compensation kit II (Art. No. F4010) must be used for the color compensation of such devices.
| Art. No. | S6125 |
|---|---|
| Test Format | 100 reactions |
| Sample Preparation | 1. DNA preparation, e.g. SureFood® PREP ANIMAL PREP or ANIMAL PREP X 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run |
| Detection Limit | The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content. |
| Shelf life | 12 month |
| Additional Information | The detection system system can be used on all available real-time PCR cyclers.ABI Prism Eppendorf realplex iCycler Rotorgene LightCycler |
SureFood® ANIMAL ID Chicken IAAC
Art. No.: S6115
Intended use:
The test detects chicken (Gallus gallus) DNA. Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).
For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
General information:
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0*, Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Applied Biossystems 7500, Bio-Rad CFX96 Agilent Mx3005P.
*note: A Color Compensation is necessary for users of the Roche LightCyclerR 2.0. The SureCC Color Compensation kit II (Art. No. F4010) must be used for the color compensation of such devices.
| Art. No. | S6115 |
|---|---|
| Test Format | 100 reactions |
| Sample Preparation | 1. DNA preparation, e.g. SureFood® PREP ANIMAL PREP or ANIMAL PREP X 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run |
| Detection Limit | The SureFood® ANIMAL ID Chicken IAAC real-time PCR is developed for the detection of chicken DNA in muscle meat mixture at a relative amount of 0.1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content. |
| Shelf life | 12 month |
| Additional Information | The detection system system can be used on all available real-time PCR cyclers.ABI Prism Eppendorf realplex iCycler Rotorgene LightCycler |
SureFood® ANIMAL ID Turkey IAAC
Intended use:
The test detects turkey (Meleagris gallopavo) DNA. Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).
For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
General information:
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm at the same time. The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler® 480, Roche LightCycler® 2.0*, Rotor-Gene Q, ABI PRISM, Bio-Rad iQ 5, Bio-Rad CFX96, Stratagene MxSeries etc.).
*note: A Color Compensation is necessary for users of the Roche LightCyclerR 2.0. The SureCC Color Compensation kit II (Art. No. F4010) must be used for the color compensation of such devices.
| Art. No. | S6116 |
|---|---|
| Test Format | 100 reactions |
| Sample Preparation | 1. DNA preparation, e.g. SureFood® PREP ANIMAL PREP or ANIMAL PREP X 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run |
| Detection Limit | The SureFood® ANIMAL ID Chicken IAAC real-time PCR is developed for the detection of chicken DNA in muscle meat mixture at a relative amount of 0.1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content. |
| Shelf life | 12 month |
| Additional Information | The detection system system can be used on all available real-time PCR cyclers.ABI Prism Eppendorf realplex iCycler Rotorgene LightCycler |
SureFood® ANIMAL QUANT Chicken
Intended use:
The test detects the relative chicken DNA amount to the total animal DNA content in meat samples. Therefore the kit contains two PCR systems, one for detection of a chicken specific gene (Gallus detection gene) and one for the detection of an animal gene (Ref reference gene).
For DNA-preparation the use of SureFood® PREP Basic is recomended.
General information:
The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler®, Rotor-Gene Q, ABI PRISM, Eppendorf realplex, BioRad CFX96, Agilent MxSeries etc.).
| Art. No. | S1014 |
|---|---|
| Test Format | 2 x 50 reactions. Thereof 50 reactions are used for the detection of the reference gene. |
| Sample Preparation | 1. DNA preparation, e.g. SureFood® PREP ANIMAL PREP (S1003) or ANIMAL PREP X (S1004) 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run |
| Detection Limit | The chicken specific PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content. The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of the reference gene are present, the relative quantitation limit for chicken DNA is 0.1 %. |
| Shelf life | 12 month |
SureFood® ANIMAL ID 4plex Pork/Chicken/Turkey+IAAC
Intended use:
The test detects pork (Sus scrofa), chicken (Gallus gallus) and turkey (Meleagris gallopavo) DNA. Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).
For DNA-preparation the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
General information:
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 522 nm, 553 nm, 610 nm and 670 nm (FAM, VIC, ROX and Cy5) at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II*, Roche cobas z 480 Analyzer*, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96 and Agilent Mx3005P.
**note: A Color Compensation is necessary for users oft the Roche LightCycler® 480 II and the Roche cobas z 480 Analyzer. For the Color Compensation of such devices the SureCC Color Compensation Kit I (Art. No. F4009) must to be used.
| Art. No. | S6123 |
|---|---|
| Test Format | 100 reactions |
| Detection Limit | The SureFood® ANIMAL ID 4plex Pork/Chicken/Turkey+IAAC real-time PCR is developed for the detection of pork, chicken and turkey DNA in muscle meat mixture at a relative amount of 0.1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content. |
| Shelf life | 12 month |
Animal Identification
DNA Preparation
Beef
Pork
Chicken / Turkey
Cat & Dog
Horse & Donkey
Fish
Rabbit