Intended use:

The SureFast® Influenza A/H9 3plex is a multiplex real-time RT-PCR for the direct, qualitative detection and
differentiation test of the flu virus influenza A and the influenza A-subtype H9.

General Information:

The detection is done in a one step real-time RT-PCR format, where the reverse transcription is followed by the
PCR in the same reaction tube. The isolated influenza A and influenza A H9 RNA is transcribed into cDNA by a
reverse transcriptase. Gene fragments specific for influenza A and influenza A H9 are subsequently amplified by
real-time PCR. The amplified targets are detected with hydrolysis probes, which are labeled at one end with a
quencher and at the other end with a fluorescent reporter dye (fluorophore). In the presence of a target the
probes hybridize to the amplicons.
The test contains an Internal Control RNA (ICR) as an internal control of sample preparation procedure and
to determine possible PCR-inhibition.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of
three fluorescence emissions at 522 nm, 553 nm and 670 nm (FAM, VIC and Cy5) at the same time (Qiagen
Rotor-Gene Q, Agilent MxSeries, Roche LightCycler® 480 etc.).

Specifications

Art. No.	F7133
Test Format	100 reactions
Shelf life	24 months