Mycotoxins’ Immunoaffinity columns (IAC) (e.g. RIDA®, EASI-EXTRACT®, PREP®) contain monoclonal antibodies making them highly selective. Further advantages are:
Excellent sample clean-up, especially with complex commodities. This makes it possible to use solvent based standards with LC-MS/MS detection.
Clean eluates mean that there is no requirement for matrix matched or isotopic labelled.
Solid phase extraction columns (SPE) use selected solid adsorbents to bind interfering components and pigments, allowing the mycotoxins to be easily analyzed. Further benefits are:
Non-specific clean-up, making this an ideal clean-up tool for the analysis of multi-toxins in simple matrices like cereals.
Simple, fast and cost-effective clean-up providing an excellent screening test in conjunction with LC-MS/MS.
Can be used in combination with matrix matched standards removing the requirement for expensive isotopic labelled standards.
HPLC and LC-MS/MS are also reference methods if it comes to legal issues. The main advantages of High Performance Liquid Chromatography (HPLC) and LC-MS/MS are their high sensitivity, applicability to complex matrixes and accuracy. However, to obtain top-notch HPLC and LC-MS/MS performance, an optimized sample clean-up using solid phase or immunoaffinity columns are required.