The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform.
The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. Aflatoxins are required to be derivatised when anal HPLC or LC-MS/MS. Aflatoxins are required to be derivatised when analysed by HPLC.
- The columns give excellent recoveries and low % RSD. Capacity and limit of detection exceed international legislation and CEN / AOAC requirements.
- Improved clean-up and concentration of toxin from complex food matrices resulting in reduced chromatography interference and lower detection limits.
- The columns are suitable for testing a wide range of commodities.
|Article Numbers||RBRDP07 / RBRP07|
|Test format||10 columns (1 ml format) (RBRDP07),
50 columns (1 ml format) (RBRP07)
|Sample preparation||A representative sample should be obtained by following one of the officially recognised sampling procedures. It is recommended that a minimum of 1 kg of representative sample is finely ground and a portion (10 – 50 g dependent on method used) of this is removed and extracted.|
|Incubation time||60 sec|
|LOD (Detection limit)||B1 0.031 ng/ml (ppt), B2 0.015 ng/ml, G1 0.015 ng/ml, G2 0.007 ng/ml|
|Validated matrices||Cereals and nuts.|
|Detected analyte||Aflatoxins B1, B2, G1 und G2 in cereals and nuts.|
|Evaluation||It is recommended to run at least a 3 – 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.|
|Approvals||CEN collaborative trials and AOAC.|