AFLAPREP®

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AFLAPREP® immunoaffinity columns for use in conjunction with HPLC or LC-MS/MS for detection of total aflatoxins in a wide range of commodities.

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Description

Intended use:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform.

 

General Information:

The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. Aflatoxins are required to be derivatised when anal HPLC or LC-MS/MS. Aflatoxins are required to be derivatised when analysed by HPLC.

Benefits:

  • The columns give excellent recoveries and low % RSD. Capacity and limit of detection exceed international legislation and CEN / AOAC requirements.
  • Improved clean-up and concentration of toxin from complex food matrices resulting in reduced chromatography interference and lower detection limits.
  • The columns are suitable for testing a wide range of commodities.

 

Accessories:

Specifications
Article Numbers RBRDP07 / RBRP07
Test format 10 columns (1 ml format) (RBRDP07),
50 columns (1 ml format) (RBRP07)
Sample preparation A representative sample should be obtained by following one of the officially recognised sampling procedures. It is recommended that a minimum of 1 kg of representative sample is finely ground and a portion (10 – 50 g dependent on method used) of this is removed and extracted.
Incubation time 60 sec
LOD (Detection limit) B1 0.031 ng/ml (ppt), B2 0.015 ng/ml, G1 0.015 ng/ml, G2 0.007 ng/ml
Validated matrices Cereals and nuts.
Detected analyte Aflatoxins B1, B2, G1 und G2 in cereals and nuts.
Evaluation It is recommended to run at least a 3 – 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.
Approvals CEN collaborative trials and AOAC.
Files
Instructions
MSDS

Additional information

Package

10 columns, 50 columns

Mycotoxins Analysis