Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of aflatoxins B1, B2, G1, G2, M1 and M2 in a wide range of commodities.
The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. Aflatoxins are required to be derivatised when analysed by HPLC. The total extraction and clean-up time takes approximately 20 minutes to perform. The result is improved clean-up and concentration of the toxins from food and feed samples giving a much cleaner chromatogram and therefore providing more accurate and sensitive detection. The columns also have the added advantage that they can be automated for large scale analysis of samples.
- The columns give excellent recoveries and low % RSD. Capacity and limit of detection exceed international legislation and CEN / AOAC requirements.
- They are suitable for testing a wide range of commodities.
- The columns are wide format allowing the sample to flow easily by gravity.
|Article Numbers||RBRRP71 / RBRRP70N|
|Test format||10 columns (3 ml format) (RBRRP71),
50 columns (3 ml format) (RBRRP70N)
|Sample preparation||A representative sample should be obtained by following one of the officially recognised sampling procedures. It is recommended that a minimum of 1 kg of representative sample is finely ground and a portion (10 – 50 g dependent on method used) of this is removed and extracted.|
|Incubation time||60 sec|
|LOD (Detection limit)||B1 0.031 ng/ml (ppt), B2 0.015 ng/ml, G1 0.015 ng/ml, G2 0.007 ng/ml|
|Validated matrices||Cereals and nuts.|
|Detected analyte||Aflatoxin B1, B2, G1 und G2 in cereals and nuts.|
|Evaluation||It is recommended to run at least a 3 – 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.|
|Approvals||The columns have been successfully used in CEN collaborative trials and methods using these columns have been approved by the AOAC.|