Vitamin B6 (Pyridoxin)
Intended use:
Easy to use microbiological microtiter plate test for the quantification of total vitamin B6 (enriched and natural vitamin B6) in food, feed and pharmaceutical products.
General Information:
Vitamin B6 is extracted from the sample and the extract is diluted. The diluted extract and the vitamin B6 assay-medium are pipetted into the wells of a microtiter plate which is coated with Saccharomyces cerevisiae. The growth of Saccharomyces cerevisiae is dependent on the supply of vitamin B6. Following the addition of vitamin B6 as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 30 °C (86 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted vitamin B6 is measured as turbidity and compared to a standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
| Art. No. | P1008 |
|---|---|
| Test Format | Microtiter plate with 96 wells (12 strips with 8 removable wells each) |
| Standard Range | 0.002 – 0.012 mg / 100 g (ml) |
| Sample Preparation | For the determination of added vitamin B6 a treatment with acid phosphatase and a subsequent heating step is necessary. For the determination of the total vitamin B6 content (native and added), the sample has to be hydrolyzed with taka diastase and acid phoshatase. |
| Incubation Time | 44 – 48 h in the dark at 30 °C (86 °F) |