AOAC-RI certified VitaFast® Vitamin B2 (Riboflavin)

Intended use:

Easy to use microbiological microtiter plate test for the quantification of total vitamin B2 (enriched and natural vitamin B2) in food, feed and pharmaceutical products.

General Information:

Vitamin B2 is extracted from the sample and the extract is diluted. The diluted extract and the vitamin B2 assay-medium are pipetted into the wells of a microtiter plate which is coated with Lactobacillus rhamnosus. The growth of Lactobacillus rhamnosus is dependent on the supply of vitamin B2. Following the addition of vitamin B2 as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted vitamin B2 is measured as turbidity and compared to a standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).

Art. No. P1007
Test Format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Standard Range 0.04 – 0.24 mg / 100 g (ml)
Sample Preparation For the determination of added and total vitamin B2 the sample has to be treated with enzymes and thereafter heat extracted.
Incubation Time 44 – 48 h in the dark at 37 °C (98.6 °F)