Vitamin B6 (Pyridoxin)
Easy to use microbiological microtiter plate test for the quantification of total vitamin B6 (enriched and natural vitamin B6) in food, feed and pharmaceutical products.
Vitamin B6 is extracted from the sample and the extract is diluted. The diluted extract and the vitamin B6 assay-medium are pipetted into the wells of a microtiter plate which is coated with Saccharomyces cerevisiae. The growth of Saccharomyces cerevisiae is dependent on the supply of vitamin B6. Following the addition of vitamin B6 as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 30 °C (86 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted vitamin B6 is measured as turbidity and compared to a standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
|Test Format||Microtiter plate with 96 wells (12 strips with 8 removable wells each)|
|Standard Range||0.002 – 0.012 mg / 100 g (ml)|
|Sample Preparation||For the determination of added vitamin B6 a treatment with acid phosphatase and a subsequent heating step is necessary. For the determination of the total vitamin B6 content (native and added), the sample has to be hydrolyzed with taka diastase and acid phoshatase.|
|Incubation Time||44 – 48 h in the dark at 30 °C (86 °F)|