Vitamin B9 (Folic Acid)
Immunoaffinity columns for use in conjuntion with an HPLC system for detection of folic acid in a wide range of commodities.
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.
|Art. No.||P81 / P81B|
|Test Format||10 wide format immunoaffinity columns (P81).
50 wide format immunoaffinity columns (P81B).
|Sample Preparation||The columns contain a gel suspension of monoclonal antibody specific to the vitamin of interest. Folic acid in the sample is extracted according to the recommended extraction procedure. The extract is then diluted with buffer, centrifuged and the supernatant filtered before being passed slowly through the immunoaffinity column where binding takes place between the antibody and the vitamin. The column is washed to remove unbound material and the vitamin is then released by the antibody following the application of elution solution. The eluate is collected prior to analysis by HPLC.|
|Incubation Time||Total: 2 hours 20 minutes.|
|Detection Limit||LOD: 10 ng/ml.|
|Shelf life||18 months at 2 – 8 °C (or 12 months at 21 – 25 °C).|
|Sensitivity||Low % CV, excellent recoveries and detection limit.|
|Rapid||Clean up 3 hours prior to detection by HPLC.|
|Robust||Can be stored at room temperature.|
|Reliable||Uses a monoclonal antibody to selectively isolate and concentrate folic acid.|
|Adaptable||Suitable for analysis of fortified folic acid. The columns are suitable for a wide range of commodities.|
|Extraction solvents||0.1 M phosphate buffer (pH 7).|
RIDASCREEN®FAST Folsäure (Folic acid) is a competitive enzyme immunoassay for the quantitative analysis of folic acid in fortified food, feed and vitamin products.
Folic acid, also known as vitamin B9, belongs to the water soluble vitamins. It is an important factor in food and also an important feed additive. Folic acid plays a key role in all growth and development processes in the body.
|Test Format||Microtiter plate with 48 wells (6 strips with 8 removable wells each)|
|Standard Range||0 ppb (zero standard), 1 ppb, 2 ppb, 5 ppb, 10 ppb, 25 ppb|
|Sample Preparation||milk: centrifugation and dilution
juices: dilution, filtration or centrifugation
other matrices: homogenization, extraction, filtration or centrifugation and dilution
|Incubation Time||25 min|
|Detection Limit||milk: approx. 10 ppb
milk powder: approx. 100 ppb
grain, cereals: approx. 100 ppb
fortified flour: approx. 400 ppb
vitamin powder, -premixes, -tablets, -capsules and -candies: approx. 1000 ppb
vitamin juice: approx. 100 ppb (corresponding to the standard substance)
|Cross Reactivity||Folic acid: 100 %
Dihydrofolic acid: 3 %
Tetrahydrofolic acid: 1.5 %
5-Methyltetrahydrofolic acid: 0.1 %
Easy to use microbiological microtiter plate test for the quantification of total folic acid (enriched and natural folic acid) in food, feed and pharmaceutical products.
Folic acid is extracted from the sample and the extract is diluted. The diluted extract and the folic acid assay-medium are pipetted into the wells of a microtiter plate which is coated with Lactobacillus rhamnosus. The growth of Lactobacillus rhamnosus is dependent on the supply of folic acid. Following the addition of folic acid as a standard or as a compound of the sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted folic acid is measured as turbidity and compared to a standard curve. The measurement is done using an microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
|Test Format||Microtiter plate with 96 wells (12 strips with 8 removable wells each)|
|Standard Range||0.16 – 1.28 µg / 100 g (ml)|
|Sample Preparation||For the determination of added folic acid in nutrient-enriched samples, a hot acid extraction is usually sufficient. However, enriched drinks can be used directly after sterile filtration and dilution. Due to the hot water extraction, natural folic acid is only measured in part. For the determination of the total folic acid content (native and added), the sample has to be hydrolyzed with enzyme.|
|Incubation Time||44 – 48 h in the dark at 37 °C (98.6 °F)|
Chicken pancreatin (γ – glutamylhydrolase) for the enzymatic treatment of food and animal feed to determine the natural folic acid content. Chicken pancreatin is used for the enzymatic treatment of food prior to the determination of its natural folic acid content. The natural folic acid is hydrolysed with chicken pancreatin into folylmonoglutamate or folyldiglutamate. Chicken pancreatin is ideal for microbiological folic acid determination in microtiter plate format (VitaFast®). It is also suitable for the determination of the natural folic acid content using other methods. The use of chicken pancreatin has proven to be particularly beneficial for green vegetables (e.g. broccoli, spinach), grain products and yeast.
|Test Format||500 mg (solid)|
|Sample Preparation||add 5 ml destillated water|
Folic acid standard for the use of spiking food, feed and pharmaceutical products.
|Test Format||3 x folic acid spiking standard (solid)|