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Quantitative

For respectively approved GMO events, there is the quantitative question of whether the product lies above the defined threshold values or mandatory labelling values.

In Europe, a value of 0.9% applies to the GMO event in the respective plant matrix. Below that value, foodstuffs with technically unavoidable and coincidental contaminations of the GMO events do not need to be declared (EC 2829/2003 und EC2830/EC). The quantification occurs via the respective quantification of a GMO specific and plant matrix specific gene based on a respectively different calibration curve and the conversion to GMO plant /matrix DNA number of copies. This dimensionless unit multiplied by 100 yields the percentage value. The calibration curve is obtained via the dilution of a DNA standard and, on the basis of a defined GMO material, can also be specified precisely with an experimental correction factor.

SureFood® GMO RoundUp Ready Soya

Intended use:

Real-time PCR for quantitative detection of RoundUp Ready Soya in food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® Prep Plant and Prep Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

The PCR product for the genetic modification has a size of 172 bp (base pairs)
( pet detection gene). The kit also contains a reference system ( lec reference gene). The PCR product has a size of 118 bp.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2014
Test Format 100 reactions
Sample Preparation 1. DNA preparation (SureFood® Prep) 2. PCR set up 3. PCR run
Detection Limit 2 copies of the target sequence for the reference and detection system each.
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® Prep)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (Filter tips) to reduce risk of contamination

SureFood® GMO Bt176 Corn

Intended use:

Real-time PCR for quantitative detection of Bt176 corn in food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® Prep Plant and Prep Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

The PCR product for the genetic modification has a size of 308 bp (base pairs)
( Cry detection gene). The kit also contains a reference system ( Zein reference gene). The PCR product has a size of 187 bp.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2015
Test Format 100 reactions
Sample Preparation 1. DNA preparation (SureFood® Prep) 2. PCR set up 3. PCR run
Detection Limit 2 copies of the target sequence for the reference and detection system each.
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® Prep)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (Filter tips) to reduce risk of contamination

SureFood® GMO Bt11 Corn

Intended use:

Real-time PCR for quantitative detection of Bt11 Corn in food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® Prep Plant and Prep Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

The PCR product for the genetic modification has a size of 190 bp (base pairs)
( ivs detection gene).). The kit also contains a reference system ( zein reference gene). The PCR product has a size of 187 bp.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2016
Test Format 100 reactions
Sample Preparation 1. DNA preparation (SureFood® Prep) 2. PCR set up 3. PCR run
Detection Limit 2 copies of the target sequence for the reference and detection system each.
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® Prep)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (Filter tips) to reduce risk of contamination

SureFood® GMO T25 Corn

Intended use:

Real-time PCR for quantitative detection of T25 Corn in food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® Prep Plant and Prep Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

The PCR product for the genetic modification has a size of 190 bp (base pairs)
( glu detection gene). The kit also contains a reference system ( zein reference gene). The PCR product has a size of 187 bp.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2017
Test Format 100 reactions
Sample Preparation 1. DNA preparation (SureFood® Prep) 2. PCR set up 3. PCR run
Detection Limit 2 copies of the target sequence for the reference and detection system each.
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® Prep)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (Filter tips) to reduce risk of contamination

SureFood® GMO MON 810 Corn

Intended use:

Real-time PCR for quantitative detection of MON 810 Corn in food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® Prep Plant and Prep Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

The PCR product for the genetic modification has a size of 282 bp (base pairs)
( hsp detection gene). The kit also contains a reference system (zein reference gene). The PCR product has a size of 187 bp.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2019
Test Format 100 reactions
Sample Preparation 1. DNA preparation (SureFood® Prep) 2. PCR set up 3. PCR run
Detection Limit 2 copies of the target sequence for the reference and detection system each.
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® Prep)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (Filter tips) to reduce risk of contamination

SureFood® GMO QUANT 35S Corn

Intended use:

Real-time PCR for quantitative detection of the 35S promotor in corn of food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® Prep Plant and Prep Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2020
Test Format 100 reactions, wherof 50 reactions are used for the quantification of the reference gene.
Sample Preparation 1. DNA preparation (SureFood® PREP) 2. PCR set up 3. PCR run
Incubation Time Depending on the amount of samples, ca. 1.5 h will be necessary for DNA extraction and 1-2 h for the PCR run.
Detection Limit 2 DNA copies
Cross Reactivity none known
Shelf life 12 month
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® PREP)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (Filter tips) to reduce risk of contamination

SureFood® GMO QUANT 35S Soya

Intended use:

Real-time PCR for quantitative detection of 35S Promotor in Soya in food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® PREP Plant and PREP Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2028
Sample Preparation 1.DNA preparation (SureFood® PREP) 2. PCR set up 3. PCR run
Incubation Time Depending on the amount of samples, ca. 1.5 h will be necessary for DNA extraction and 1-2 h for the PCR run.
Detection Limit 2 DNA copies
Cross Reactivity none known.
Shelf life 12 month
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® PREP)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (Filter tips) to reduce risk of contamination

SureFood® GMO 35S Soya

Intended use:

Real-time PCR for quantitative detection of 35S Promotor in Soya in food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® PREP Plant and PREP Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2028
Test Format 100 reactions
Sample Preparation 1.DNA preparation (SureFood® PREP) 2. PCR set up 3. PCR run
Incubation Time Depending on the amount of samples, ca. 1.5 h will be necessary for DNA extraction and 1-2 h for the PCR run.
Detection Limit 2 DNA copies
Cross Reactivity none known
Shelf life 12 month
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® PREP)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (Filter tips) to reduce risk of contamination

SureFood® GMO RR2Y Soya

Intended use:

Real-time PCR for quantitative detection of RR2Y Soya (RoundUp Ready 2 Yield Soya) in food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® PREP Plant and PREP Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2029
Test Format 100 reactions
Sample Preparation 1. DNA preparation (SureFood® PREP) 2. PCR set up 3. PCR run
Detection Limit ≤ 5 DNA copies
Shelf life 12 month
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® Prep)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (Filter tips) to reduce risk of contamination

SureFood® GMO QUANT MIR162 Corn

Intended use:

The test detects the relative quantitative MIR162-Corn DNA amount.

Specifications
Art. No. S2135
Test Format 2 x 50 Reactions
Sample Preparation For DNA-preparation of raw material the use of SureFood® PREP Plant and for highly processed food and
feed the use of SureFood® PREP Plant X is recommended.
Detection Limit The MIR162-Corn PCR has a limit of detection of ≤ 5 DNA-copies.
Shelf life 12 month
Additional Information The real-time PCR assay can be used with established real-time PCR
instruments (Roche LightCycler, Rotor-Gene Q, ABI PRISM, Eppendorf realplex, BioRad CFX96, Stratagene
MxSeries etc.).