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Screen Test

A screening of plant extrinsic gene sequences that were infiltrated into the plants during GMO development provides efficient evidence of the presence of any possible GMOs.

Combination possibilities as screening kits:

S2126 4plex (35S/NOS/FMV +IAC)
This multiplex kit enables the screening of the most important parameters within a reaction, but requires multiplex-capable devices that can separately detect FAM/VIC/ROX/Cy5 (Agilent MX3005P, ABI 7500, Biorad CFX96, RotorgeneQ, Roche Lightcycler 480).

S2026 (35S+NOS+FMV)
This kit enables screening of the same parameters only in the FAM channel. Here though, four separated reaction procedures and tubes have to be used for the three parameters and for an external inhibition control.

These and further parameters can either be combined in a modular way or used individually. The efficiency of the DNA preparation can be verified with a plant-specific PLANT Plus Kit (s2049). The PLANT Kit (S2056) is sufficient for the 4plex Kit with internal amplification control.

SureFood® GMO P35S:BAR Rice Screening

Intended use:

Real-time PCR for qualitative detection of P35S:BAR rice DNA in food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® PREP Plant and PREP Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2022
Test Format 100 reactions, whereas 50 reactions are used for detection of the reference gene.
Sample Preparation 1. DNA preparation (SureFood® PREP) 2. PCR set up 3. PCR run
Incubation Time Depending on the amount of samples approximately 1.5 h for DNA extraction and 1-2 h for the PCR run.
Detection Limit 2 DNA copies
Cross Reactivity none known
Shelf life 12 month
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® Prep)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (Filter tips) to reduce risk of contamination

 

SureFood® GMO SCREEN 35S + NOS + FMV

Intended use:

Real-time PCR for qualitative detection of 35S Promotor plus NOS Terminator plus FMV in food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® PREP Plant and PREP Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2026
Test Format 3 x 100 reactions
Sample Preparation 1. DNA preparation (SureFood® PREP) 2. PCR set up 3. PCR run
Incubation Time Depending on the amount of samples, ca. 1.5 h will be necessary for DNA extraction and 1-2 h for the PCR run.
Detection Limit 2 DNA copies
Cross Reactivity none known
Shelf life 12 month
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® PREP)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (Filter tips) to reduce risk of contamination

 

SureFood® GMO SCREEN CaMV

Intended use:

Detection of the Cauliflower mosaic virus.
DNA vectors of this virus are used in GMO events and the detection/exclusion of the virus may verify positive GMO detection.
Test kits for DNA preparation are separately available (SureFood® PREP Plant and PREP Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed with all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2027
Test Format 50 reactions
Sample Preparation 1. DNA preparation (SureFood® PREP) 2. PCR set up 3. PCR run
Detection Limit < 5 DNA copies
Shelf life 12 month
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® PREP)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (filter tips) to reduce risk of contamination

 

SureFood® GMO SCREEN 35S

Intended use:

Real-time PCR for qualitative detection of 35S DNA in food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® PREP Plant and PREP Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed with all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2045
Test Format 50 reactions
Sample Preparation 1. DNA preparation (SureFood® PREP) 2. PCR set up 3. PCR run
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® PREP)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (filter tips) to reduce risk of contamination

 

SureFood® GMO SCREEN NOS

Intended use:

Real-time PCR for qualitative detection of NOS DNA in food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® PREP Plant and PREP Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed with all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2046
Test Format 50 reactions
Sample Preparation 1. DNA preparation (SureFood® PREP) 2. PCR set up 3. PCR run
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® PREP)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (filter tips) to reduce risk of contamination

 

SureFood® GMO SCREEN FMV

Intended use:

Real-time PCR for qualitative detection of FMV DNA in food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® PREP Plant and PREP Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed with all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2047
Test Format 50 reactions
Sample Preparation 1. DNA preparation (SureFood® PREP) 2. PCR set up 3. PCR run
Shelf life 12 month
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® PREP)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (filter tips) to reduce risk of contamination

 

SureFood® GMO SCREEN BAR

Intended use:

Real-time PCR for qualitative detection of BAR DNA in food, feed and environmental samples. Test kits for DNA preparation are separately available (SureFood® PREP Plant and PREP Plant X).

PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® GMO real time PCR kits use FAM/TAMRA labelled TaqMan probes.

PCR protocols are available for:
LightCycler (Roche)
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
iCycler (Bio-Rad)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)

The PCR kit can be performed with all commonly used real-time PCR Cyclers.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2048
Test Format 50 reactions
Sample Preparation 1. DNA preparation (SureFood® PREP) 2. PCR set up 3. PCR run
Shelf life 12 months
Additional Information Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® PREP)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (filter tips) to reduce risk of contamination

 

SureFood® GMO PLANT PLUS

Intended use:

The test detects plant DNA. Each reaction contains an internal amplification control (PLUS). The real-time
PCR assay can be used with established real-time PCR instruments, equipped for detection of two
fluorescence emissions in the FAM and VIC/HEX channel at the same time.

For users of the Roche LightCycler 1.5 and 2.0 a color compensation is necessary. The use of the SureCC Color Compensation Kit II
(S4010) is recommended

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0,9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2049
Test Format 100 reactions
Sample Preparation For DNA-preparation of raw material the use of SureFood® PREP Plant and for highly processed food
and feed the use of SureFood® PREP Plant X is recommended.
Detection Limit The SureFood® GMO Plant PLUS PCR has a limit of detection of ≤ 5 DNA copies

 

SureFood® GMO Plant

Intended use:

This kits detects plant DNA and serves as an additional modul for SureFood® GMO 4plex 35S/NOS/FMV/IAC (S2126) or SureFood® GMO Screen 35S+NOS+FMV (S2026). This kit does not contain and internal amplification control and may be used with all commercial available real-time PCR thermocyclers.

Specifications
Art. No. S2056
Test Format 100 reactions
Sample Preparation The use of the DNA preparation kit SureFood® PREP Plant (S1002) for raw material and SureFood® PREP Plant X (S1006) for highly processed food is recommended.
Detection Limit ≤ 5 DNA copies
Shelf life 12 month

 

SureFood® GMO SCREEN CTP2:CP4 EPSPS

Intended use:

This sequence denotes the transition from chloroplast transit peptide to 5-enolpyruvylshikimate-3-phosphate synthase from Arabidopsis thaliana und Agrobacterium spp. It enables a resistance against the herbicide glyphosate.
This sequence might be used for a directed screening approach.

General Information:

The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90’s. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0.9 % GMO for food and feed. In accordance with § 64 of the LfGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.

Specifications
Art. No. S2059
Test Format 100 reactions
Sample Preparation 1. DNA preparation (SureFood® PREP Plant S1002 or PREP Plant X S1006)
2. PCR set up
3. PCR run
Detection Limit ≤ 5 DNA copies depending on the matrix and processing grade.
Shelf life 12 month

 

SureFood® GMO SCREEN 4plex 35S/NOS/FMV/IAC

Intended use:

Screening for the GMO promotors or terminators is usally the first step for GMO analysis. This assay allows in a single reaction a differential screening. Positive samples may be subsequentially analysed for qualitative and quantitative event specific GMO.

General Information:

The analysis requires a real-time thermocyler equipped with four detection channels for emission of fluorescence at 522nm, 553 nm, 610 nm, and 670 nm (FAM, VIC, ROX, Cy5) as:
Biorad CFX96
Stratagene MX series
Rotor-Gene Q
Roche Lightcycler® 480

The Lightcycler® 480 needs to be adjusted with the colour compensation I kit (S4009).

Specifications
Art. No. S2126
Test Format 100 reactions
Sample Preparation The use of the DNA preparation kit SureFood® PREP Plant (S1002) for raw material and SureFood® PREP Plant X (S1006) for highly processed food is recommended. Special matrices as e.g. honey requires a special sample preparation.
Detection Limit ≤ 5 DNA copies
Shelf life 12 month