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Sesame

 

Lateral Flow Sesame

 

Intended use:

The Lateral Flow Sesame assay is a qualitative detection assay of sesame residues in surface samples got from e.g. production lines. The assay is also applicable for food samples. The assay is applied for the detection of small amounts of sesame contaminations.

General Information:

Sesame contaminations in food possibly elicit hypersensitivity in sensitive persons. Food production lines have to be free of residues to prevent these allergic reactions. The effectiveness of cleaning can be verified by using the Lateral Flow Sesame swabbing method.

Specifications
Art. No. BL609-10
BL609-25
Test Format 10 x test strips (1 determination per strip)
25 x test strips (1 determination per strip)
Sample Preparation Swabbing method: cleaning surface with pretreated swab, wash out the swab and measure
Food assay: homogenise food sample, filter, measure
Incubation Time 10 min
Detection Limit Swabbing method: 10µg dried sample on surface
Food assay (zero matrix): 1 ppm
External link I http://www.bioavid.de/

 

 

RIDASCREEN®FAST Sesame

 

Intended use:

RIDASCREEN®FAST Sesame (Art. No. R7202) is a sandwich enzyme immunoassay for the quantitative analysis of sesame or parts of sesame (as ingredient components or contamination) in instant soup, chocolate dessert, bakery products and crackers.

Specifications
Art. No. R7202
Test Format Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range 0 ppm (zero standard), 2.5 ppm, 5 ppm, 10 ppm, 20 ppm almond
Sample Preparation homogenization, extraction and centrifugation
Incubation Time 30 min
Detection Limit 0.14 mg/kg (ppm) sesame (0.08 – 0.2 depending on the matrix)
Cross Reactivity The polyclonal antibody specifically detects proteins from almonds. Cross reaction to apricot stone is > 100 %. No cross-reaction with cashew nut, brazil nut, pecan, hazelnut, coconut, macadamia nut, walnut, chest nut, sunflower seeds, sesame and lima beans.

SureFood® ALLERGEN Sesame – NEW

 

Intended use:

The test detects sesame DNA according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.

For the quantitative determination the use of the laboratory reference material  SureFood® QUANTARD Allergen 40 containing 40 mg sesame / kg food sample and a detection system including a standard dilution is required.

For DNA preparation, the use of SureFood® PREP Advance, protocol 1, is recommended.

General Information:

The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent Mx3005P.

Specifications
Art. No. S3608
Test Format Test-Kit with 100* reactions
Sample Preparation 1. DNA preparation, e.g. SureFood® PREP ALLERGEN 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run
Detection Limit Depending on the sample matrix, processing grade, DNA preparation and DNA contant, the detection is limited to < 5 DNA copies / ≤ 0. 4 ppm.
Shelf life 12 month
Additional Information The detection system system can be used on all available real-time PCR cyclers.ABI Prism
Eppendorf realplex
iCycler
Rotorgene
LightCycler

*Includes Internal Amplification Control (IAC) in VIC/HEX channel. SureFood®QUANTARD Allergen 40 must be used for quantifiction.