Lateral Flow Sesame
The Lateral Flow Sesame assay is a qualitative detection assay of sesame residues in surface samples got from e.g. production lines. The assay is also applicable for food samples. The assay is applied for the detection of small amounts of sesame contaminations.
Sesame contaminations in food possibly elicit hypersensitivity in sensitive persons. Food production lines have to be free of residues to prevent these allergic reactions. The effectiveness of cleaning can be verified by using the Lateral Flow Sesame swabbing method.
|Test Format||10 x test strips (1 determination per strip)
25 x test strips (1 determination per strip)
|Sample Preparation||Swabbing method: cleaning surface with pretreated swab, wash out the swab and measure
Food assay: homogenise food sample, filter, measure
|Incubation Time||10 min|
|Detection Limit||Swabbing method: 10µg dried sample on surface
Food assay (zero matrix): 1 ppm
|External link I||http://www.bioavid.de/|
RIDASCREEN®FAST Sesame (Art. No. R7202) is a sandwich enzyme immunoassay for the quantitative analysis of sesame or parts of sesame (as ingredient components or contamination) in instant soup, chocolate dessert, bakery products and crackers.
|Test Format||Microtiter plate with 48 wells (6 strips with 8 removable wells each)|
|Standard Range||0 ppm (zero standard), 2.5 ppm, 5 ppm, 10 ppm, 20 ppm almond|
|Sample Preparation||homogenization, extraction and centrifugation|
|Incubation Time||30 min|
|Detection Limit||0.14 mg/kg (ppm) sesame (0.08 – 0.2 depending on the matrix)|
|Cross Reactivity||The polyclonal antibody specifically detects proteins from almonds. Cross reaction to apricot stone is > 100 %. No cross-reaction with cashew nut, brazil nut, pecan, hazelnut, coconut, macadamia nut, walnut, chest nut, sunflower seeds, sesame and lima beans.|
SureFood® ALLERGEN Sesame – NEW
The test detects sesame DNA according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg sesame / kg food sample and a detection system including a standard dilution is required.
For DNA preparation, the use of SureFood® PREP Advance, protocol 1, is recommended.
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent Mx3005P.
|Test Format||Test-Kit with 100* reactions|
|Sample Preparation||1. DNA preparation, e.g. SureFood® PREP ALLERGEN 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run|
|Detection Limit||Depending on the sample matrix, processing grade, DNA preparation and DNA contant, the detection is limited to < 5 DNA copies / ≤ 0. 4 ppm.|
|Shelf life||12 month|
|Additional Information||The detection system system can be used on all available real-time PCR cyclers.ABI Prism
*Includes Internal Amplification Control (IAC) in VIC/HEX channel. SureFood®QUANTARD Allergen 40 must be used for quantifiction.