Hepatitis A detection by real-time PCR
The test is a real-time RT-PCR for the direct and qualitative detection of hepatitis A virus. The test contains
an Internal Control RNA (ICR) as an internal control of sample preparation procedure and to determine
possible PCR-inhibition. The real-time PCR assay can be used with established real-time PCR instruments,
equipped for detection of two fluorescence emissions at 522 nm and 553 nm (FAM and VIC) at the same
time (Rotor-Gene Q, Stratagene Agilent MxSeries, Roche LightCycler® 480/2.0*/1.5* etc.).
|Test Format||100 reactions|
|Sample Preparation||For RNA-preparation the use of SureFast® PREP DNA/RNA Virus is recommended.|
|Detection Limit||The SureFast® VIRUS Hepatitis A PLUS real-time RT-PCR has a limit of detection of ≤ 50 RNA copies. The
assay limit of detection depends on RNA preparation and RNA content.
|Shelf life||24 month|