The test is a multiplex real-time RT-PCR for the direct and qualitative detection of Norovirus and Hepatitis A
virus. The test contains an Internal Control RNA (ICR) as an internal control of sample preparation
procedure and to monitor possible PCR-inhibition. The real-time RT-PCR assay can be used with real-time
PCR instruments, equipped for detection of three fluorescence emissions at 522 nm, 553 nm and 670 nm
(FAM, VIC, Cy5) at the same time (Rotor-Gene Q, Stratagene MxSeries, Roche LightCycler® 480 etc.).
|For DNA-preparation the use of SureFast® PREP DNA/RNA Virus is recommended.
|SureFast® VIRUS Norovirus/Hepatitis A 3plex real-time RT-PCR has a limit of detection of ≤ 50 RNA
copies. The assay limit of detection depends on RNA preparation and RNA content.