Intended use:

Salmonella detection with real-time PCR

Two in one – Salmonella test kit with included DNA preparation

The kit is intended to be used for the fast and simple isolation and detection of Salmonella – DNA from
enrichment cultures.
Each reaction contains an internal amplification control (PLUS). The real-time PCR assay can be used with
established real-time PCR instruments, equipped for detection of two fluorescence emissions in the FAM and
VIC/HEX channel at the same time. The technical validation of instruments was performed on Agilent
Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche LightCycler® 2.0, Applied Biosystems 7500,
Qiagen RotorGene Q and Cepheid SmartCycler®.

Art. No. F5211
Test Format 100 reactions
Sample Preparation thermal lysis without additional steps
Detection Limit The SureFast® Salmonella ONE real-time PCR has a limit of detection of ≤ 5 DNA copies (1 cfu after pre-enrichment). The assay limit of
detection depends on sample matrix, processing grade, DNA preparation and DNA content.
Shelf life 24 months