Salmonella detection with real-time PCR
Two in one – Salmonella test kit with included DNA preparation
The kit is intended to be used for the fast and simple isolation and detection of Salmonella – DNA from
Each reaction contains an internal amplification control (PLUS). The real-time PCR assay can be used with
established real-time PCR instruments, equipped for detection of two fluorescence emissions in the FAM and
VIC/HEX channel at the same time. The technical validation of instruments was performed on Agilent
Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche LightCycler® 2.0, Applied Biosystems 7500,
Qiagen RotorGene Q and Cepheid SmartCycler®.
|Test Format||100 reactions|
|Sample Preparation||thermal lysis without additional steps|
|Detection Limit||The SureFast® Salmonella ONE real-time PCR has a limit of detection of ≤ 5 DNA copies (1 cfu after pre-enrichment). The assay limit of
detection depends on sample matrix, processing grade, DNA preparation and DNA content.
|Shelf life||24 months|