Extraction and purification of Salmonella DNA from bacterial cultures. Official standard methods are recommended for pre-enrichment of samples (ISO, DIN, §64 LFBG) to meet requirements of official regulations.
SureFood® is a modular system consisting of DNA preparation, real-time PCR detection. Each module is separately available.
SureFood® PREP uses the spin column technique. The DNA extract obtained can be used for PCR detection directly without further treatment. Please refer to SureFood® PATHOGEN Salmonella PLUS for DNA detection.
Salmonella is a genus of rod-shaped Gram-negative enterobacteria that causes typhoid fever, paratyphoid fever and foodborne illness. It is motile in nature and produces hydrogen sulfide.
Disease-causing salmonellae have recently been re-classified into a single species, Salmonella enterica, which has numerous strains or serovars. Other salmonellae are frequent causes of foodborne illness, and can especially be caught from poultry and raw eggs and more generally from food that has been cooked or frozen, and not eaten straight away. In March 2006, the U.S. government reported that 16.3% of all chickens were contaminated with salmonella. In the mid to late 20th century, Salmonella enterica serovar Enteritidis was a common contaminant of eggs. This is much less common now with the advent of hygiene measures in egg production and the vaccination of laying hens to prevent salmonella colonisation.
|Test Format||2 x 50 reactions|
|Sample Preparation||1. Aliquot from bacterial culture (pre-enrichment) 2. Lysis by boiling (99°C) 3. Centrifugation of the lysed sample 4. Adjustment of optimal binding conditions 5. Loading of spin filter and binding of the DNA 6. Washing of the bound DNA 7. Elution of the DNA from the spin filter|
|Additional Information||Depending on the expected yield of DNA, the elution buffer volume can be adapted.
Minimum volume for elution is 50 µl. The eluted DNA is ready-to-use for the PCR.
The DNA can be stored for up to 24 hours at 4°C. For a storage time of
more than 24 hours it should be kept at -20°C or –70°C.
Materials required but not provided with the test kit:
• Reagents for pre-enrichment