Easy to use microbiological microtiter plate test for the quantification of total inositol (added and native inositol) in food, animal feed and pharmaceutical products.
Inositol is extracted from the sample and the extract is diluted. The diluted extract and the inositol assay – medium are pipetted into the wells of a microtiter plate which is coated with Saccharomyces cerevisiae. The growth of Saccharomyces cerevisiae is dependent on the supply of inositol. Following the addition of inositol as a standard or as a compound of the sample, the bacteria grow until the inositol is consumed. The incubation is done in the dark at 30 °C (86 °F) for 44 – 48 h. The intensity of metabolism or growth of Saccharomyces cerevisiae in relation to the extracted inositol is measured as turbidity and compared to a standard curve. The measurement is done using a microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
|Test Format||Microtiter plate with 96 wells (12 strips with 8 removable wells each)|
|Standard Range||0.5 – 5.0 mg / 100 g|
|Sample Preparation||For the determination of total inositol content, the
sample has to be treated by enzymatic extraction
|Incubation Time||44 – 48 h in the dark at 30 °C (86 °F)|