Vitamin B7 (Biotin)
Intended use:
Immunoaffinity columns for use in conjuntion with an HPLC or LC-MS/MS system for detection of biotin in a wide range of commodities.
General Information:
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.
| Art. No. | P82 / P82B |
|---|---|
| Test Format | 10 wide format immunoaffinity columns (P82). 50 wide format immunoaffinity columns (P82B). |
| Sample Preparation | The columns contain a gel suspension of monoclonal antibody specific to the vitamin of interest. Biotin in the sample is extracted according to the recommended extraction procedure. The extract is then diluted with buffer, centrifuged and the supernatant filtered before being passed slowly through the immunoaffinity column where binding takes place between the antibody and the vitamin. The column is washed to remove unbound material and the vitamin is then released by the antibody following the application of solvent. The sample is then evaporated and reconsituted prior to analysis by HPLC or LC-MS/MS. |
| Detection Limit | LOD: 25 ng/ml |
| Shelf life | 18 months at 2 – 8 °C (or 12 months at 21 – 25 °C). |
| Sensitivity | Low % CV, excellent recoveries and detection limit. |
| Rapid | Clean-up 3 hours prior to detection by HPLC. |
| Robust | Can be stored at room temperature. |
| Reliable | Uses a specific monoclonal antibody to selectively isolate and concentrate biotin. |
| Adaptable | Suitable for analysis of total biotin. The columns are suitable for a wide range of commodities. |
| Extraction solvents | Methanol or Acetonitrile. |
| Email Link | biotica@otenet.gr |
Intended use:
RIDASCREEN® Biotin is a competitive enzyme binding assay for the quantitative analysis of added biotin in vitaminized food and vitamin products. The enzymatic sample preparation for milk and milk powder allows the determination of the total biotin content (added and natural biotin).
For the detection of Biotin in serum samples a special kit is available under Art. No.: H1601.
General Information:
Biotin, also known as vitamin H, is an important nutritional factor and food additive, playing a major role in the metabolism of both humans and animals alike. Biotin acts as a coenzyme and participates in the process of gluconeogenesis, biosynthesis of fatty acids and the metabolism of amino acids. Biotin deficiency in mammals leads to growth inhibition, loss of appetite and reduced utilization of food components. Advanced stages of avitaminosis manifest themselves in hair loss, dermatitis and signs of paralysis. The biotin requirement for adults is between 30 and 60 µg per day.
| Art. No. | R2201 |
|---|---|
| Test Format | Microtiter plate with 96 wells (12 strips with 8 removable wells each) |
| Standard Range | 0 ppt (zero standard), 37 ppt, 111 ppt, 333 ppt, 1000 ppt |
| Sample Preparation | homogenization, (optional digestion with a-amylase) heating, centrifugation, (optional Ascorbase-treatment) and dilution |
| Incubation Time | 1 h 30 min |
| Detection Limit | milk: approx. 5 ppb milk powder: approx. 55 ppb grain products: approx. 3.7 ppb vitamin powder etc.: approx. 370 ppt vitamin juice: approx. 370 ppt(corresponding to the standard substance) |
| Cross Reactivity | The specificity of the RIDASCREEN® Biotin test was determined by analysing the cross reactions to corresponding substances in buffer.Biotin: 100 % Biotin-4-Amidobenzoat: approx. 89 % Biocytin: approx. 83 % |
Intended use:
Easy to use microbiological microtiter plate test for the quantification of total biotin (enriched and natural biotin) in food, feed and pharmaceutical products.
General Information:
Biotin is extracted from the sample and the extract is diluted. The diluted extract and the biotin assay-medium are pipetted into the wells of a microtiter plate which is coated with Lactobacillus plantarum . The growth of Lactobacillus plantarum is dependent on the supply of biotin. Following the addition of biotin as a standard or as a compound of the
sample, the bacteria grow until the vitamin is consumed. The incubation is done in the dark at 37 °C (98.6 °F) for 44 – 48 h. The intensity of metabolism or growth in relation to the extracted biotin is measured as turbidity and compared to a standard curve. The measurement is done using an microtiter plate spectrophotometer at 610 – 630 nm (alternatively at 540 – 550 nm).
| Art. No. | P1003 |
|---|---|
| Test Format | Microtiter plate with 96 wells (12 strips with 8 removable wells each) |
| Standard Range | 0.08 – 0.72 µg / 100 g (ml) |
| Sample Preparation | For the determination of added biotin in nutrient-enriched samples, a hot water alkali extraction is usually sufficient. However, enriched drinks can be used directly after sterile filtration and dilution. Due to the hot water extraction, natural biotin is only measured in part. For the determination of the total biotin content (native and added) of most foods (with the exception of cereals), the sample has to be treated with enzyme. For cereal samples with native biotin content, the sample must be extracted by autoclaving. |
| Incubation Time | 44 – 48 h in the dark at 37 °C (98.6 °F) |
Intended use:
Biotin – standard for the use of spiking food, feed and pharmaceutical products.
| Art. No. | P3003 |
|---|---|
| Test Format | 3 x biotin spiking standard (solid) |