Intended use:
The kit allows the sensitive and group specific detection and partial differentiation between the beer spoilage microorganisms in pre-incubated beer or beverage samples:
Lactobacillus/ Pediococcus/Pectinatus/Megasphaera/yeasts
The detection requires a DNA preparation. This may be performed using the GEN-IAL® Simplex® Easy DNA kit.
The screening and differentiation kit is designed for the use with the ABI 7500, Agilent MX 3005P and Aria MX, Biorad CFX96, Roche Lightcycler® 480, Qiagen/Rotorgene Q amd other suitable devices with FAM/VIC-HEX/ROX/Cy5 detection channels and is based on the TaqMan™ technology.
General Information:
The beer spoilage micro organisms of the group of Lactobacillus, Pediococcus, Pectinatus, Megasphaera, or wild yeasts may influence the beer aroma and taste. To detect these specialized beer spoilage micro organisms quickly during production or after filling, the real-time kit serves as a quick screening kit.
Comment:
The kit PP1T 0050 replaces the former kits: PP1T 0050 LC480 for Lightcycler® 480; PP1T 0050 RG for Rotorgene Q, PP1T 0048 ABI for ABI 7500.
| Art. No. | PP1T 0050 |
|---|---|
| Test Format | 48 reactions |
| Sample Preparation | The use of the GEN-IAL® Simplex® Easy DNA kit (SE 0100) or for worth and very difficult samples the GEN-IAL® First-Beer Magnetic DNA kit (FBD 0100) is recommended. |
| Incubation Time | A preincuabtion for 48 hours in a suitable medium (as NBB or MRS) is recommended. The optimal pre-incubation condition must be validated indivdually at the brewery. |
| Shelf life | 6 months |