FENICOLS
Chloramphenicol is a broad spectrum fenicol antibiotic which is frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties. However, in humans it leads to hematotoxic side effects, in particular chloramphenicol-induced aplastic anaemia for which a dosage-effect relationship has not yet been established.
RIDASCREEN® Chloramphenicol
Intended use:
RIDASCREEN® Chloramphenicol is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in milk, milk powder and dairy products, honey and royal jelly, meat, fish, shrimp, eggs, urine (also chloramphenicol glucuronide), plasma/serum and feed.
General Information:
Chloramphenicol is a broad spectrum antibiotic which is frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties. However, in humans it leads to hematotoxic side effects, in particular chloramphenicol-induced aplastic anaemia for which a dosage-effect relationship has not yet been established. This has led to a prohibition of chloramphenicol for the treatment of animals used for food production.
Accessories:
- RIDA® Chloramphenicol Spiking Solution
| Art. No. | R1511 |
|---|---|
| Test format | Microtiter plate with 96 wells (12 strips with 8 removable wells each) |
| Incubation Time | 45 min |
| Detection Limit | milk : 24 ppt milk powder: 25 ppt yogart/kefir/buttermilk/cream: 12 ppt curd/sour cream: 15 ppt butter:61 ppt cheese: 16ppt honey: 25 ppt royal jelly: 23 ppt meat: 5 ppt fish/shrimps: 8 ppt egg: 15 ppt urine: 196 ppt plasma/serum: 18 ppt feed: 107 ppt |
RIDA® Chloramphenicol Spiking Solution
Art. No.: R1599
Intended use:
RIDA® Chloramphenicol Spiking Solution can be used for the production of positive control samples suitable for the validation (e.g. determination of the recovery rate) of the RIDASCREEN® Chloramphenicol test (R1511).
| Art. No. | R1599 |
|---|---|
| Test Format | Concentration: 50 ng/ml Volume: 1 ml Chloramphenicol in buffer, ready to use |
EASI-EXTRACT® CHLORAMPHENICOL
Art. No.: P300 / P300B
Intended use:
Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS system for detection of chloramphenicol in a wide range of commodities.
The columns contain a gel suspension of monoclonal antibody specific to the antibiotic of interest. The sample is diluted with buffer, vortexed (or placed on an orbital shaker), filtered and passed slowly through the immunoaffinity column. Any antibiotic which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the antibiotic is then released from the column following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC or LC-MS/MS.
General Information:
he procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.
| Art. No. | P300 / P300B |
|---|---|
| Test Format | 10 wide format immunoaffinity columns (P300). 50 wide format immunoaffinity columns (P300B). |
| Sample Preparation | The columns contain a gel suspension of monoclonal antibody specific to the antibiotic of interest. The sample is diluted with buffer, vortexed (or placed on an orbital shaker), filtered and passed through the immunoaffinity column. Any antibiotic which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the antibiotic is then released by the antibody following elution with solvent. The sample is then evaporated and reconstituted prior to analysis by HPLC or LC-MS/MS. |
| Detection Limit | HPLC: 20 ng/ml of chloramphenicol. LC-MS/MS: 0.02 ng/ml of chloramphenicol. |
| Shelf life | 2 years at 2 – 8 °C (or 12 months at 21 – 25 °C). |
| Sensitivity | Low % CV, excellent recoveries, capacity and limit of detection exceeds the minimum residue performance limit when used in conjunction with LC-MS/MS. |
| Rapid | Clean up in 20 minutes prior to detection by HPLC or LC-MS/MS. |
| Robust | Can be stored at room temperature. |
| Reliable | Uses a specific monoclonal antibody to selectively isolate and concentrate chloramphenicol. |
| Adaptable | The columns are suitable for testing a wide range of commodities. |
| Extraction solvents | Methanol. |
| Email Link | biotica@otenet.gr |