Polypeptide antibiotics are a chemically diverse class of anti-infective and antitumor antibiotics containing non-protein polypeptide chains. Examples of this class include actinomycin, bacitracin, colistin, and polymyxin B.
Bacitracin is a mixture of different polypeptides, wherein bacitracin A as major component has the highest biological activity.
Colistin A and B are polypeptide antibiotics that have been used in medicine and veterinary for over 50 years.
RIDASCREEN® Bacitracin is a competitive enzyme immunoassay for the quantitative analysis of bacitracin in milk, meat, eggs, feed and urine
Bacitracin belongs to the group of polypeptide antibiotics. It was named after the Bacillus strain producing it and the 7 year old girl ‘Tracy’ from whose open tibial fracture the strain was isolated in 1945 for the first time. Bacitracin is a mixture of different polypeptides, wherein bacitracin A as major component has the highest biological activity. Through inhibition of cell wall synthesis, it has a broad spectrum bactericidal effect against both gram-positive and gram-negative bacteria.
In addition to veterinary application, bacitracin can be used as antimicrobial growth promoter in animal husbandry. Thereby, residues can remain in foodstuff of animal origin and may pose a health risk to consumers. Improper use of antibiotics also promotes the formation of antibiotic resistant bacteria, which raise an increasing health problem for the population. Consequently, Bacitracin has been deleted by the regulation 2821/98/EC from the list of approved animal feed additives (Directive 70/524/EEG Appendix B) and maximum residue limits in foodstuffs have been set (Regulation 37/2010/EC).
|Test format||Microtiter plate with 96 wells (12 strips with 8 removable wells each)|
|Sample preparation||milk: pH-adjustment, defatting, dilution
meat, eggs and feed: homogenization, extraction, centrifugation, dilution
|Incubation time||1.5 h|
|Detection limit||milk: approx. 11 µg/kg (ppb)
meat: approx. 9 µg/kg (ppb)
eggs: approx. 11 µg/kg (ppb)
feed: approx. 82 µg/kg (ppb)
urine : approx. 23 µg/kg (ppb)(corresponding to the standard substance)
Bacitracin (standard substance) : 100%
Virginiamycin: < 3 %
Tylosin: < 2 %
Spiramycin, Neomycin, Colistin: < 1 %
A competitive enzyme immunoassay for screening and quantitative analysis of colistin.
Colistin A and B are polypeptide antibiotics that have been used in medicine and veterinary for over 50 years. The European Medicines Agency has proposed to reduce the use of the antibiotic drug colistin in food producing animals. For several food matrices maximum residue limits (MRLs) for colistin are set in the European Union: 150 μg/kg for muscle, fat and liver; 200 μg/kg for kidney, 50 μg/kg for milk; 300 μg/kg for eggs.
The Colistin ELISA is a competitive enzyme immunoassay based on specific antibodies directed against colistin.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For milk, egg, chicken, pork, beef, liver, fish and feed fast and efficient extraction methods are included in the kit manual.|
|Incubation time||60 minutes|
|Detection limit||Milk 4 ppb
Eggs 22 ppb
Chicken 12 ppb
Pork 8 ppb
Beef 15 ppb
Liver 21 ppb
Fish 12 ppb
Feed 24 ppb
|Cross reactivity||Colistin A and B 100%, Polymyxin B1 and B2 100%, Bacitracin <0.1%|