In 1948, aureomycin (chlortetracycline) was isolated by Duggan as a metabolite of the actinomyces species Streptomyces aureofaciens. This was the first antibiotic substance of the group of tetracyclines.
Worldwide, tetracycline, chlortetracycline and oxytetracycline are authorized for veterinary use. Therefore, health risks for the consumer can occur. In all animal species which are used for food production, tetracycline residues (the sum of mother substance and the 4-epimer) are limited in the EU law, commission regulation (EU) No 37/2010 with the following limits (MRLs): 100 ppb in muscle and in milk. The RIDASCREEN® Tetracyclin test can be used to detect tetracycline in milk and dairy products (cheese, butter, curd, yoghurt, kefir, cream, sour cream), meat and meat products as well as seafood, egg and honey.
Art. No.: R3505
RIDASCREEN® Tetracyclin is a competitive enzyme immunoassay for the quantitative analysis of tetracyclins in milk, milk powder, cheese, butter, dairy products (curd, yoghurt (plain/with fruits), kefir, cream, sour cream) honey, meat, sausage, fish, shrimp and whole egg.
1948 aureomycin (chlortetracycline) was isolated by Duggan as a metabolite of the actinomyces species streptomycec aureofaciens. This was the first antibiotic substance of the group of tetracyclines. In Germany tetracycline, chlortetracycline and oxytetracycline are authorized for veterinary use. Therefore health risks for the consumer can occur. In all animal species which are used for food production tetracycline residues (the sum of mother substance and the 4-epimer) are limited in the EU law, commission regulation (EU) No 37/2010 with the following limits: 100 ppb in muscle and in milk.
- RIDA® Tetracyclin Spiking Solution R3599
|Test Format||Microtiter plate with 96 wells (12 strips with 8 removable wells each)|
|Standard Range||0 ppb (zero standard), 0.5 ppb,1.5 ppb, 3 ppb, 6 ppb, 18 ppb (concentrates)|
|Sample Preparation||milk: dilution
milk powder: dissolving and dilution
cheese: homogenization and degreasing
meat: homogenization and extraction
shrimps: homogenization and extraction
|Incubation Time||1 h 30 min|
|Detection Limit||milk: approx. 1 ppb
milkpowder: approx. 21 ppb
cheese: approx. 4 ppb
honey: approx. 4 ppb
meat: approx. 2 ppb
shrimps: approx. 1.5 ppb
butter*: approx. 3 ppb
dairy products*: approx. 1 ppb
sausage*: approx. 5 ppb
whole egg*: approx. 3 ppb(corresponding to the standard substance)* Application available on request
|Cross Reactivity||The specificity of the RIDASCREEN® Tetracyclin test was established by analyzing the cross-reactivity to corresponding antibiotics in buffer system:
Tetracycline: 100 %
RIDA® Tetracyclin /Spiking Solution
RIDA® Tetracyclin Spiking Solution can be used for the production of positive control samples suitable for the validation (e.g. determination of the recovery rate) of the RIDASCREEN® Tetracyclin test (R3505).
|Test format||Tetracycline lyophilisate (1 μg tetracycline hydrochlorid)
after reconstitution: 10 ml with a final concentration of 100 ng/ml
A competitive enzyme immunoassay for screening and quantitative analysis of oxytetracyclines.
Tetracyclines are a group of antibiotics derived from Streptomyces spp. with a broad-spectrum activity.The most commonly used tetracyclines in veterinary medicine are tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC) and doxycycline (DC). In many countries in the world legislation has become in force to control the use of OTC in aquacultural products like salmon, pangasius and shrimps. Furthermore, the use of OTC in the honey industry has to be controlled.
The Oxytetracycline ELISA is a competitive enzyme immunoassay based on antibodies directed against oxytetracycline.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For honey and shrimps/fish fast and efficient methods are included in the kit manual.|
|Incubation time||90 minutes|
|Detection limit||Honey 5 ppb
Shrimps/fish 2 ppb
|Cross reactivity||Oxytetracycline 100%, Tetracycline 133%, Chlortetracycline 138%, Doxycycline 54%|