Art. No.: P10
A simple membrane test for the rapid detection of non-durum wheat adulteration of durum wheat (semolina).
Durum wheat (also known as Triticum durum) is the ideal raw material for pasta production and the economically most important wheat species. Since prices for durum wheat are usually higher than prices for non-durum wheat, grain products are sometimes adulterated with non-durum wheat.
The kit uses a monoclonal antibody which is specific for the protein friabilin which is present in non-durum wheats but not in durum wheat.
|Test format||Reagents and membrane strips suitable for 40 analysis.|
|Sample preparation||Flour samples are extracted to release any friabilin protein. The extract is then absorbed onto the membrane, and the strip is saturated with a blocking agent. An enzyme labelled monoclonal antibody specific to the protein is then added. If non-durum wheat is present in the sample then a complex will be formed between the extracted friabilin protein and the enzyme labelled antibody. After washing the strip, a clear coloured staining reagent is added which forms a blue / purple coloured deposit in the presence of the friabilin /antibody enzyme complex. The degree of blue / purple colour with the sample is proportional to the percentage of non-durum wheat present. No colour developed indicates 100 % durum wheat (i.e. no adulteration).|
|Incubation time||The total assay time takes approximately 35 minutes to perform.|
|Detection limit||Detects 3 % non-durum wheat.|
|Approvals||Tested in a BPPA, BCR, MAFF trial for durum wheat authenticity.|