Gestagens (also known as progestogens or progestagens) are female sex hormones and a class of steroid hormones. They are sometimes used as growth promoters in livestock.Synthetic gestagens can be used for estrus inhibition or synchronisation after break off and improvement of fertility.When animals are treated with gestagens, drug residues may remain in the animal’s meat and milk. With our test systems, you can reliably detect gestagen residues.
RIDASCREEN® Acetylgestagene is a competitive enzyme immunoassay for the quantitative analysis of medroxyprogesterone acetate (MPA) and other acetylgestagenes in bovine perirenal fat.
- RIDA® Acetylgestagene Spiking Solution
|Test format||Microtiter plate with 96 wells (12 strips with 8 removable wells each)|
RIDA® Acetylgestagene Spiking Solution
RIDA® Acetylgestagene Spiking Solution can be used for the production of positive control samples suitable for the validation (e.g. determination of the recovery rate) of the RIDASCREEN® Acetylgestagene test (R1801).
|Test format||medroxyprogesteronacetat in methanol
concentration: 100 ng/ml
volume: 1 ml
RIDASCREEN® Melengestrolacetat is a competitive enzyme immunoassay for the quantitative analysis of melengestrol acetate in bovine perirenal fat and muscle meat. The performance of the test is adjusted to the current legal demands and well suited for the control of meat exported by the USA and Canada.
Synthetic gestagens can be used for estrus inhibition or synchronisation after break off and improvement of fertility. In addition one of these substances, melengestrol acetate (MLGA), is a licensed growth promoting feed additive for heifers in the USA and Canada. The admitted dose is 0.5 mg per day and head, given as a feed premix. Its mode of anabolic action is unclear, but it stimulates the ovarian synthesis of the endogenous anabolic steroid estradiol and that may have androgenic side effects. MLGA belongs to the most active synthetic gestagens. Its oral bioactivity is about 10 or 100 times higher than the activities of the gestagens chlormadinone acetate (CMA) or medroxyprogesterone acetate (MPA), resp. If given parenterally the hormonal activity of MLGA is still 125 times higher than the one of progesterone. Due to its strongly lipophilic properties, MLGA is accumulated in fat 200-fold higher than in blood plasma. In the European Union the use of sexual hormones for growth promoting purposes is generally forbidden since 1988, and also the import of meat from hormone treated cattle into the EU is prohibited.
- RIDA® Melengestrolacetat Spiking Solution
|Test Format||Microtiter plate with 48 wells (6 strips with 8 removable wells each)|
|Standard Range||0 ppb (zero standard), 0.15 ppb, 0.45 ppb, 1.35 ppb, 4.05 ppb, 12.15 ppb|
|Sample Preparation||extraction over night centrifugation, evaporation, freezing, centrifugation and chromatographic purification with RIDA® C18 column (Art. No.: R2002)|
|Incubation Time||2 h 30 min|
|Detection Limit||bovine perirenal fat: 0.3 ppb
muscle meat: 0.075 ppb
|Cross Reactivity||Melengestrol acetate (MLGA): 100 %
Megestrol acetate (MGA): approx. 10 %
Medroxyprogesterone acetate (MPA): approx. 6 %
6-α-Methyl-17-α-OH-Progesterone acetate: approx. 5
Chlormadinone acetate (CMA): approx. 5 %
17-α-Acetoxyprogesterone: approx. 4 %
16-α-Methylprogesterone: 0.0035 %
cross reaction of all other substances being checked in the assay is less 0.003 %
RIDA® Melengestrolacetat Spiking Solution
RIDA® Melengestrolacetat Spiking Solution can be used for the production of positive control samples suitable for the validation (e.g. determination of the recovery rate) of the RIDASCREEN® Melengestrolacetat test (R6502).
A spiking procedure for different matrices, which are listed in the product leaflet of the RIDASCREEN® Melengestrolacetat test is available on request.
|Test format||melengestrolacetate in methanol
concentration: 100 ng/ml
volume: 1 ml