QUINOLONES/ FLUROQUINOLONES
The Quinolones/ Fluroquinolones (chinolones, flumequine, fluoroquinolones) are a family of synthetic broad-spectrum antibiotic drugs. They inhibit the gyrase enzyme and are therefore also known as gyrase inhibitors.
Gyrase inhibitors are divided into four subgroups. The majority of quinolones belong to the subgroup of fluoroquinolones, which have a fluoro-group attached at the central ring system, typically at the 6th position. The fluoroquinolones belong to the so called second generation quinolones. Some of these are admitted as antibiotics in veterinary medicine for food producing animals. Fluoroquinolones are broad spectrum antibiotics against a lot of bacterial species. They are used frequently in veterinary medicine especially for cattle, pigs and chicken. The usage of fluoroquinolones has increased in the past years because large amounts of these antibiotics were applied to prevent infectious diseases, especially in chicken, swine and fish/shrimp farming. The wide use in food producing animals has generated microbial resistances. This led to an amendment of Council Regulation (EEC) No 2377/90 and in the introduction of MRLs (Maximum Residue Limits) for some fluoroquinolones.
RIDASCREEN®Chinolone/Quinolones
Intended use:
RIDASCREEN® Chinolone is a competitive enzyme immunoassay for the quantitative analysis of chinolones in egg, beef, pork, sheep, chicken, turkey, fish and shrimp.
General information:
Gyrase inhibitors are devided into four subgroups. The majority of chinolones belong to the subgroup of fluorochinolones, which have a fluoro-group attached at the central ring system, typically at the 6th position. The fluorchinolones belong to the so called second generation chinolones. Some of these are admitted as antibiotics in veterinary medicine for food producing animals. Fluorchinolones are broad spectrum antibiotics against a lot of bacterial species. They are used frequently in veterinary medicine especially for cattle, pigs and chicken. The usage of fluorchinolone has increased in the past years because large amounts of fluorchinolones were applied to prevent infectious diseases, especially in chicken, swine and fish/shrimp farming. The wide use in food producing animals has generated microbial resistances. This led to an amendment of Council Regulation (EEC) No 2377/90 and in the introduction of MRLs (Maximum Residue Limits) for some fluorchinolones
| Art. No. | R3113 |
|---|---|
| Test Format | Microtiter plate with 96 wells (12 strips with 8 removable wells each) |
| Standard Range | 0 ppb (zero standard), 0.5 ppb, 1.5 ppb, 3 ppb, 6 ppb, 18 ppb |
| Sample Preparation | egg, shrimps: homogenization, extraction, centrifugation meat, fish: homogenization, extraction, centrifugation, dilution |
| Incubation Time | 1 h 15 min |
| Detection Limit | shrimps: 6 ppb fish: 8 ppb egg: 9 ppb meat: 10 ppb raw milk*: 0.5 ppb (corresponding to the standard substance) * Application on request |
| Cross Reactivity | Ciprofloxacin: 100 % Norfloxacin, Enrofloxacin, Marbofloxacin, Danofloxacin, Difloxacin, Flumequin, Ofloxacin: > 100 % Sarafloxacin: 43 % Oxolinic acid: 24 % |
RIDA® Ciprofloxacin Spiking Solution
Intended use:
RIDA® Ciprofloxacin Spiking Solution can be used for the production of positive control samples suitable for the validation (e.g. determination of the recovery rate) of the RIDASCREEN® Chinolone/Quinolones test (R3113).
A spiking procedure for different matrices, which are listed in the product leaflet of the RIDASCREEN® Chinolone/Quinolones test is available on request.
| Art. No. | R3198 |
|---|---|
| Test Format | concentration: 1 μg/ml volume: 1 ml ciprofloxacin in buffer, ready-to-use |
EuroProxima Flumequine
Intended use:
A competitive enzyme immunoassay for screening and quantitative analysis of flumequine in various matrices.
General information:
Flumequine is a second-generation Quinolone antibiotic and is mainly active against Gram negative bacteria. In Europe the MRLs for Flumequine in the different matrices depend on the matrix and the species from which the matrix origins. MRLs vary from 200 μg/kg (muscle) to 1500 μg/kg (kidney). The antiserum used in the ELISA is specific for Flumequine and does not cross-react with any of the other Fluoroquinolones tested. For detection of most Fluoroquinolones in one test we advise the generic Fluoroquinolones ELISAs (5101FLUQG and 5101FLUQGII).
The Flumequine ELISA is a competitive enzyme immunoassay based on antibodies directed against flumequine.
| Art. No. | 5101FLUM |
|---|---|
| Test format | Microtiter plate, 96 wells. |
| Sample preparation | For milk, serum, meat, shrimp, egg, urine, feed, honey and water fast and efficient methods are included in the kit manual. |
| Incubation time | 90 minutes |
| Detection limit | Milk 13 ppb Serum 1 ppb Meat <0.1 ppb Shrimp <0.1 ppb Egg 3.5 ppb Urine 5.5 ppb Feed 10 ppb Honey <10 ppb Water 3 ppb |
| Cross reactivity | Flumequine 100%, All other fluoroquinolones tested < 0.1% |
EuroProxima Fluoroquinolones I
Intended use:
A competitive enzyme immunoassay for screening and quantitative analysis of a broad range of fluoroquinolones in various matrices.
General information:
Fluoroquinolones are a synthetic class of antibiotics which all act by inhibition of bacterial DNA-gyrase. The antiserum used in the ELISA is directed against the main Fluoroquinolones including Enrofloxacin and its metabolite Ciprofloxacin. In combination with our specific ELISA against Flumequine nearly all Fluoroquinolones can be screened for in a fast and reliable way.
The Fluoroquinolones ELISA is a competitive enzyme immunoassay based on antibodies directed against fluoroquinolones.
| Art. No. | 5101FLUQG |
|---|---|
| Test format | Microtiter plate, 96 wells. |
| Sample preparation | For milk, egg, tissue, cheese, honey, feed, water, serum and urine fast and efficient methods are included in the kit manual. |
| Incubation time | 90 minutes |
| Detection limit | Milk 3 ppb Egg 6 ppb Tissue and whole egg 0.5 ppb Soft and hard cheese 6 ppb Honey 2 ppb Feed 5 ppb Water 2 ppb Tissue 0.3 ppb Serum 2.5 ppb Urine 7 ppb |
| Cross reactivity | Ciprofloxacin 124%, Norfloxacin 100%, Enrofloxacin 92%, Danofloxacin 89%, Nadifloxacin 85%, Pefloxacin 70%, Piromidic acid 62%, Oxolinic acid 57%, Enoxacin 57%, Lomefloxacin 40%, Fleroxacin 40%, Ofloxacin 18%, Marbofloxacin 16%, Pipemidic acid 5%, Gatifloxacin 5%, Sarafloxacin 4%, Levofloxacin 3%, Flumequine 2%, Difloxacin 1%, Pazufloxacin 1% |
EuroProxima Fluoroquinolones II
Intended use:
A competitive enzyme immunoassay for screening and quantitative analysis of fluoroquinolones in various matrices.
General information:
With the fluoroquinolones ELISA (5101FLUQG) we introduced the first “generic” fluoroquinolones ELISA. With our new broad-spectrum kit (5101FLUQII) we fulfil the increased demands from the (Asian) market. In the table the cross-reactivities of the antibodies used in the respective assay are presented.
The Fluoroquinolones II ELISA is a competitive enzyme immunoassay based on antibodies directed against fluoroquinolones.
| Art. No. | 5101FLUQII |
|---|---|
| Test format | Microtiter plate, 96 wells. |
| Sample preparation | For shrimps, porcine muscle, tissue, honey, serum, urine and feed fast and efficient methods are included in the kit manual. |
| Incubation time | 45 minutes |
| Detection limit | Shrimps 4 ppb Porcine muscle 6 ppb Tissue 0.6 ppb Honey 0.1 ppb Serum 3 ppb Urine 1.5 ppb Feed 16 ppb |
| Cross reactivity | Norfloxacin 100%, Enrofloxacine 84%, Ciprofloxacin 91%, Enoxacin 119%, Ofloxacin 62%, Fleroxacin 92%, Marbofloxacin 113%, Sarafloxacin 92%, Difloxacin 105%, Levofloxacin 173%, Danofloxacin 47%, Tosufloxacin 67%, Gatifloxacin 16% |