Tetracyclines

 

In 1948, aureomycin (chlortetracycline) was isolated by Duggan as a metabolite of the actinomyces species Streptomyces aureofaciens. This was the first antibiotic substance of the group of tetracyclines.

Worldwide, tetracycline, chlortetracycline and oxytetracycline are authorized for veterinary use. Therefore, health risks for the consumer can occur. In all animal species which are used for food production, tetracycline residues (the sum of mother substance and the 4-epimer) are limited in the EU law, commission regulation (EU) No 37/2010 with the following limits (MRLs): 100 ppb in muscle and in milk. The RIDASCREEN® Tetracyclin test can be used to detect tetracycline in milk and dairy products (cheese, butter, curd, yoghurt, kefir, cream, sour cream), meat and meat products as well as seafood, egg and honey.

RIDASCREEN® Tetracyclin

Art. No.: R3505

Intended use:

RIDASCREEN® Tetracyclin is a competitive enzyme immunoassay for the quantitative analysis of tetracyclins in milk, milk powder, cheese, butter, dairy products (curd, yoghurt (plain/with fruits), kefir, cream, sour cream) honey, meat, sausage, fish, shrimp and whole egg.

General information:

1948 aureomycin (chlortetracycline) was isolated by Duggan as a metabolite of the actinomyces species streptomycec aureofaciens. This was the first antibiotic substance of the group of tetracyclines. In Germany tetracycline, chlortetracycline and oxytetracycline are authorized for veterinary use. Therefore health risks for the consumer can occur. In all animal species which are used for food production tetracycline residues (the sum of mother substance and the 4-epimer) are limited in the EU law, commission regulation (EU) No 37/2010 with the following limits: 100 ppb in muscle and in milk.

Accessories:

  • RIDA® Tetracyclin Spiking Solution R3599
Specifications
Art. No. R3505
Test Format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Standard Range 0 ppb (zero standard), 0.5 ppb,1.5 ppb, 3 ppb, 6 ppb, 18 ppb (concentrates)
Sample Preparation milk: dilution
milk powder: dissolving and dilution
cheese: homogenization and degreasing
honey: dilution
meat: homogenization and extraction
shrimps: homogenization and extraction
Incubation Time 1 h 30 min
Detection Limit milk: approx. 1 ppb
milkpowder: approx. 21 ppb
cheese: approx. 4 ppb
honey: approx. 4 ppb
meat: approx. 2 ppb
shrimps: approx. 1.5 ppb
butter*: approx. 3 ppb
dairy products*: approx. 1 ppb
sausage*: approx. 5 ppb
whole egg*: approx. 3 ppb(corresponding to the standard substance)* Application available on request
Cross Reactivity The specificity of the RIDASCREEN® Tetracyclin test was established by analyzing the cross-reactivity to corresponding antibiotics in buffer system:

Tetracycline: 100 %
Chlortetracycline: approx. 70 %
Rolitetracycline: approx. 34 %
Demeclocycline: approx. 26 %
Oxytetracycline: approx. 13 %
Minocycline: approx. 3 %
Doxycycline: approx. 2 %

 

RIDA® Tetracyclin /Spiking Solution

Art. No.: R3599

Intended use:

RIDA® Tetracyclin Spiking Solution can be used for the production of positive control samples suitable for the validation (e.g.  determination of the recovery rate) of the RIDASCREEN® Tetracyclin test (R3505).

Specifications
Art. No. R3599
Test format Tetracycline lyophilisate (1 μg tetracycline hydrochlorid)
after reconstitution: 10 ml with a final concentration of 100 ng/ml

 

 

EuroProxima Oxytetracycline

Art. No.: 5091OTC

Intended use:

A competitive enzyme immunoassay for screening and quantitative analysis of oxytetracyclines.

General information:

Tetracyclines are a group of antibiotics derived from Streptomyces spp. with a broad-spectrum activity.The most commonly used tetracyclines in veterinary medicine are tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC) and doxycycline (DC). In many countries in the world legislation has become in force to control the use of OTC in aquacultural products like salmon, pangasius and shrimps. Furthermore, the use of OTC in the honey industry has to be controlled.

The Oxytetracycline ELISA is a competitive enzyme immunoassay based on antibodies directed against oxytetracycline.

Specifications
Art. No. 5091OTC
Test format Microtiter plate, 96 wells.
Sample preparation For honey and shrimps/fish fast and efficient methods are included in the kit manual.
Incubation time 90 minutes
Detection limit Honey 5 ppb
Shrimps/fish 2 ppb
Cross reactivity Oxytetracycline 100%, Tetracycline 133%, Chlortetracycline 138%, Doxycycline 54%