SureFood® ANIMAL ID Pork SENS PLUS

Intended use:

The test detects pork DNA (Sus scrofa). Each reaction contains an internal amplification control.

General Information:

The realtime PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 510 nm and 580 nm (FAM and VIC/HEX) at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, BioRad CFX 96, Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Roche LightCycler® 2.0 Applied Biosystems 7500, LTF MyGo Pro, R-BIOPHARM RIDA®CYCLER and Qiagen RotorGene Q.

Comment:

Many conventional produced meat products show an unavoidable cross contamination when pork and beef or poultry is produced on a shared production line. Although not detectable with common systems, these contaminations can be detected by using SureFood® ANIMAL ID Pork SENS. Currently there is no internationally harmonized threshold for a non declaration of pork content of food. In many European countries an undeclared pork amount of 0.5 to 1 % or less is mostly accepted by the governmental authorities (the detection limit of SureFood® ANIMAL ID Pork SENS is far below that).

In other region of the world different acceptable level of contamination might be accepted. These countries must find practically acceptable thresholds for pork content since unavoidable contaminations can be detected with novel analytical methods like SureFood® ANIMAL ID Pork SENS.

The test contains an internal inhibition control (PLUS).