Chicken pancreatin (γ-glutamylhydrolase) for the enzymatic treatment of food and animal feed to determine the natural folic acid content. Chicken pancreatin is used for the enzymatic treatment of food prior to the determination of its natural folic acid content. The natural folic acid is hydrolysed with chicken pancreatin into folylmonoglutamate or folyldiglutamate. Chicken pancreatin is ideal for microbiological folic acid determination in microtiter plate format (VitaFast®). It is also suitable for the determination of the natural folic acid content using other methods.