RIDASCREEN® Clenbuterol is a competitive enzyme immunoassay for the quantitative analysis of clenbuterol in milk, meat, liver, kidney, urine, plasma/serum, hair, eyeball and feed.
Clenbuterol belongs to the group of β-agonists. It has been known that β-agonists are suitable for use as performance improvers within the field of livestock production. In particular, the meat/fat ratio in fattened animals can be improved or the growth accelerated. However, such compounds have not been authorized as fattening adjuvants up to now.
In addition to its lipolytic and anabolic effect, clenbuterol has a relaxing effect on non-striated musculature, on which its therapeutic employment as an antiasthmatic and a tocolytic agent is based. In comparison to therapeutic use clenbuterol is administered at 5 to 10 time’s higher dosages, when employed as a fattening adjuvant. Therefore, it is possible that clenbuterol residues, after use in illegal practice, may lead to a risk for consumers.
- Direct use of milk, urine and plasma/ serum samples in the test without sample preparation.
- Less pipetting through functionalized microtiter plate.
- No cross reactivity to other relevant ß-agonists such as ractopamine or zilpaterol.
- RIDA® ß-Agonists & Clenbuterol Spiking Solution
- RIDA® Clenbuterol Assay Control (positive) for RIDASCREEN® Clenbuterol (R1711)
- RIDA® Clenbuterol Assay Control (negative) for RIDASCREEN® Clenbuterol (R1711)
- RIDA® Sample Decolorant
- RIDASOFT® Win.NET Food & Feed
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||Milk: direct use,
meat: homogenization, extraction, evaporation,
liver: homogenization, extraction, treatment with RIDA® Sample Decolorant (Art. No. R1699),
kidney: homogenization, extraction,
urine, plasma/serum: direct use, hair: washing, boiling,
eyeball: dissection, extraction,
feed: grinding, extraction, treatment with RIDA®Sample Decolorant (Art. No. R1699).
|Incubation time||45 min|
|LOD (Detection limit)||Milk approx. 50 ng/l (ppt), meat approx. 100 ng/kg, liver approx. 150 ng/kg, kidney approx. 200 ng/kg, urine approx. 100 ng/l, plasma/serum approx. 250 ng/l, hair approx. 2 μg/kg (ppb), eyeball approx. 200 ng/kg, feed approx. 600 ng/kg|
|Validated matrices||Milk, meat, liver, kidney, urine, plasma/serum, hair, eyeball and feed.|
|Detected analyte||Clenbuterol in milk, meat, liver, kidney, urine, plasma/serum, hair, eyeball and feed.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|