Description
Intended use:
Quantitative determination of high molecular weight ß-Glucan from barley in malt-mash and wort (EBC 4.16.3, MEBAK 3.1.4.9.2), in combination with sample extraction method EBC 4.5.1. The assay does not involve any enzyme, the reaction is a chemical / colorimetric reaction (therefore it belongs ot the product line Enzytec™ Color).
Two test-kits are available for respectively 40 tests (Art. No. E3500) and 160 tests (Art. No. E3550).
General Information:
The determination of the ß-Glucan concentration is important for the malt and beer industry.
The test has been developed by the company NovaBiotec – Dr. Fechter GmbH and production has been transferred to R-Biopharm AG. The raw materials and the production procedures for the original GlucaTest® Rapid assay (NovaBiotec) and the new Enzytec Color Gluca Test kits are identical. Only the packaging has been slightly changed in order to fit to the new production facilities.
Benefits:
- Easy: Principle of photometric test; without special sample preparation; without additional laboratory equipment.
- Fast: Results after 30 minutes reaction time & quasi-online process control is possible.
- Favorable: Savings compared to conventional methods of analysis.
- Reliable: High correlation between photometric and fluorometric methods.
| Article Numbers | E3500 / E3550 |
|---|---|
| Test format | • GlucaTest S125 (E3500) = 40 tests (1 x 125 ml solution A + calibrator set), • GlucaTest L500 (E3550) = 160 tests (4 x 125 ml solution A + calibrator set) |
| Sample preparation | The method for sample preparation has been described under EBC method 4.5.1 (Extract of malt: Congress mash). It is mandatory to prepare the samples according to this method if the test is used according to EBC 4.16.3. |
| Incubation time | 30 minutes |
| Validated matrices | barley in malt-mash and wort |
| Detection range | 100 – 500 mg/L ß-Glucan |
| Detected analyte | ß-Glucan |
| Evaluation | Colorimetric assay (550 nm) |
| Instructions | |
|---|---|
| MSDS |
