EuroProxima Carazolol is a competitive enzyme immunoassay for screening and quantitative analysis of carazolol in various matrices.
It is a common practice for farmers to use a tranquilliser or β-blocker such as carazolol to prevent stress of pigs f. e. during transportation. It is a nonspecific β-adrenergic blocking agent that is used especially in pigs; a less widespread use is in the prevention of reproductive stress in dairy cows. In the EU, maximum residue limits (MRLs) have been established for both pigs and cattle. The compound is listed in Annex I of Council Regulation (EEC) No. 2377/90. The marker residue is the parent substance.
The EuroProxima Carazolol ELISA is a competitive enzyme immunoassay based on antibodies directed against carazolol.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||For mentioned matrices fast and efficient extraction methods are included in the kit description.|
|Incubation time||1 h 30 min (20 – 25 °C)|
|LOD (Detection limit)||Tissue 0.3 ng/ml (ppb), liver 3 ng/ml, urine 2.2 ng/ml, kidney 3 ng/ml|
|Detection capability CCβ||Tissue 0.3 ng/ml|
|Validated matrices||Tissue, liver, urine and kidney.|
|Detected analyte||Carazolol in tissue, liver, urine or kidney.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|