A competitive enzyme immunoassay for screening and quantitative analysis of lincomycin in various matrices.
Lincomycin is a narrow spectrum antibiotic derived from Streptomyces lincolnensis. It belongs to the lincosamide group which also includes pirlimycin and clindamycin. The EU has set maximum residue limits (MRLs) for lincomycin in different foods of animal origin: 50 μg/kg (ppb) for fat/eggs, 100 μg/kg for muscle, 150 μg/kg for milk, 500 μg/kg for liver and 1500 μg/kg for kidney. Although in the EU no MRLs have been established for antibiotics in honey, some countries including Belgium, Hungary, Switzerland and the United Kingdom have set Action Limits for lincomycin, which vary from 10 to 50 μg/kg.
The Lincomycin ELISA is a competitive enzyme immunoassay based on specific antibodies directed against lincomycin.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||For the mentioned matrices, fast and efficient methods are included in the test kit description.|
|Incubation time||1 h 30 min|
|LOD (Detection limit)||Milk 45 μg/l, milk (sensitive) 0.6 μg/l, tissue 41 μg/kg, liver 100 μg/kg, honey 7 μg/kg, egg 20 μg/kg|
|Detection capability CCβ||Milk 75 μg/l, milk (sensitive)1 μg/l, tissue 50 μg/kg, liver 250 μg/kg, honey 10 μg/kg, egg 25 μg/kg|
|Validated matrices||Milk, tissue, liver, honey and egg.|
|Detected analyte||Lincomycin in various matrices|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|