EuroProxima Nortestosterone is a competitive enzyme immunoassay for screening and quantitative analysis of 19-Nortestosterone in various matrices.
Anabolic preparations are attractive means of improving the growth rate and feed conversion of animals in livestock breeding. In all European Community countries however, the use of growth promoters has been banned since 1986. The anabolic steroid 19-nortestosterone is one of the most frequently found growth promoters. The European Community allows a limit of 2 ng/ml (ppb) 19-nortestosterone in bile, faeces and urine.
In most EC countries urine is chosen as the matrix to screen for the presence of growth promoters. In the urine of most species anabolics and their (hydroxy) metabolites are mainly excreted as conjugates of glucuronic and sulphuric acids. For the determination of the analytes in urine from treated animals, (enzymatic) hydrolysis of the conjugates to free steroids is necessary.
Endogenous 19-nortestosterone occurs in urine of male pigs and horses.
With this ELISA it is possible to screen a large amount of urine samples in a short time for the presence of 17β-19-nortestosterone and its major metabolites (17α-19-nortestosterone and 19-norandrostendione).
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||For mentioned matrices fast and efficient extraction methods are included in the kit description.|
|Incubation time||1 h 30 min (2 – 8 °C / 20 – 25 °C)|
|LOD (Detection limit)||Urine 0.5 µg/l (ppb), milk 0.5 µg/l|
|Validated matrices||Urine and milk.|
|Detected analyte||17β-19-nortestosterone and its major metabolites (17α-19-nortestosterone and 19-norandrostenedione) in urine and milk.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|