EuroProxima Plus Cow’s Whey is a competitive enzyme immunoassay for the detection of cow’s rennet whey in whey from other species.
The EuroProxima Plus Cow’s Whey ELISA uses a specific antibody raised against the glycomacropeptide (GMP) part of bovine milk protein ĸ-casein. The binding of this antibody to the ĸ-casein-coated microtiter plate is inhibited by any bovine GMP present in the sample. Bovine GMP is released from cow’s ĸ-casein present in milk during cheese production and it is present
in cow’s rennet whey fraction.
Thus, the Plus Cow’s Whey ELISA is a fast screening tool for the detection of cow’s rennet whey residues in whey products from other species. The test can be used for such
- Quality control of the incoming goods, for example for the detection of residues of cow’s rennet whey in goat/sheep’s whey batch before a batch is processed further.
- Quality control of a final product, for example for the detection of residues of cow’s rennet whey powder in goat/sheep’s whey powder as a result of cross-contamination between production batches.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||For mentioned matrices fast and efficient extraction methods are included in the kit description.|
|Incubation time||45 min (20 – 25 °C)|
|LOD (Detection limit)||0.1-0.3 %|
|Detection capability CCβ||0.5 %|
|Validated matrices||Whey (of different species)|
|Available application notes||5171WHEY app04.21|
|Detected analyte||Bovine glycomacropeptide (GMP) part of κ-casein.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|