This competitive enzyme immunoassay is used for the quantitative determination of trenbolone in urine, liver and tissue.
Trenbolone acetate is a very efficient anabolic steroid associated with a strong androgenic activity. When used correctly, no genotoxic activity of trenbolone has been shown so far. Nevertheless, trenbolone in elevated concentrations causes cell transformations in embryonic cells of the golden hamster and reacts weakly positive in the Ames test.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||Hydrolysis, chromatographic pre-cleaning with RIDA® C18 column (Art. No.: R2002).|
|Incubation time||1 h 30 min|
|LOD (Detection limit)||Urine 0.5 μg/l (ppb), liver 0.6 μg/kg, tissue 0.4 μg/kg|
|Detection capability CCβ||Urine 0.7 μg/l, liver 0.7 μg/kg, tissue 0.5 μg/kg|
|Validated matrices||Urine, liver and tissue.|
|Detected analyte||Trenbolone in urine, liver and tissue.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|