A qualitative screening card for detection of ochratoxin A at various levels in food and feed commodities.
The kit is based on monoclonal antibody technology which has the advantage of being highly specific and sensitive while the test format is rapid and simple to perform. The screening procedure is intended to serve as an indicator of the presence of toxins at various screening levels according to European and International legislation.
The toxin is extracted from the sample, filtered and passed through an immunoaffinity column before being diluted and applied to the card. The conjugate is applied to the membrane and unbound conjugate is then removed by washing. A colourless substrate is added and the card is incubated for five minutes. Finally a stop solution is applied to the membrane. A purple spot must appear at the control site to indicate that the test is valid. A purple spot at the sample site shows that the contamination is less than the cut off value of the card. No colour at the sample site indicates contamination at a higher level than the cut off of the card.
|Test format||10 cards containing 20 tests and controls|
|Sample preparation||A representative sample should be obtained by following one of the officially recognised sampling procedures. It is recommended that a minimum of 1 kg of representative sample is finely ground and a portion (10 – 50 g dependent on method used) of this is removed and extracted.|
|Incubation time||15 minutes|
|LOD (Detection limit)||3-10 µg/kg (ppb)|
|Validated matrices||Food and feed.|
|Available application notes||Methods are available for all matrices covered by legislation as well as additional commodities. Deviation from the methods described in our Instructions For Use and Application Notes may not result in optimum results. Please contact your local R-Biopharm distributor for further information.|
|Detected analyte||Ochratoxin A in food and feed commodities.|
|Evaluation||Visual evaluation: The control spot must develop a clearly visible purple colour in order to have a valid test result. The colour in the sample and the control spot does not need to be of the same intensity. If doubt arises regarding the presence of a spot it is advised to hold the card at arm’s length (against a dark background). If the spot can be clearly seen then it can be considered as being present.|