OCHRARHONE® immunoaffinity columns for use in conjunction with HPLC or LC-MS/MS for detection of ochratoxin A in a wide range of commodities.
The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform.
The columns contain a gel suspension of monoclonal antibody specific to the toxin of interest. Following extraction of the toxin the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS.
The total extraction and clean-up time takes approximately 20 minutes to perform. The result is improved clean-up and concentration of the toxin from food and feed samples giving a much cleaner chromatogram and therefore providing more accurate and sensitive detection. The columns also have the added advantage that they can be automated for large scale analysis of samples.
- Can be used for the analysis of certain metabolites and masked mycotoxins.
- The columns are wide format allowing the sample to flow easily by gravity.
|Article Numbers||RBRP59/25 /- RBRP59/100|
|Test format||25 columns (1 ml format) (RBRP59/25),
100 columns (1 ml format) (RBRP59/100)
|Sample preparation||A representative sample should be obtained by following one of the officially recognised sampling procedures. It is recommended that a minimum of 1 kg of representative sample is finely ground and a portion (10 – 50 g dependent on method used) of this is removed and extracted.|
|Incubation time||60 sec|
|LOD (Detection limit)||0.05 ng/ml (ppt)|
|Validated matrices||Cereals and dried fruit.|
|Available application notes||Methods are available for all matrices covered by legislation as well as additional commodities. Please contact your local R-Biopharm distributor for further information.|
|Detected analyte||Ochratoxin A in cereals and dried fruit.|
|Evaluation||It is recommended to run at least a 3 – 6 point calibration curve. In constructing a suitable curve the levels of the calibration standards should bracket or include the range of expected results. The diluted standard solutions should be prepared fresh on the day of analysis and used within a 24 hour period.|