SureFast® EHEC/EPEC 4plex is a real-time PCR for the direct, qualitative detection and differentiation of DNA sequences of the virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the E. coli and Shigella spp. virulence factor ipaH.
The test contains an Internal Control DNA (ICD) as an internal control of sample preparation procedure and to determine possible PCR-inhibition.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 522 nm, 553 nm, 610 nm und 670 nm (FAM, VIC, ROX und Cy5) at the same time. The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Applied Biosystems 7500 and Cepheid SmartCycler®.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFast® PREP Bacteria is recommended.|
|LOD (Detection limit)||The SureFast® EHEC/EPEC 4plex real-time PCR has a limit of detection of ≤ 5 DNA copies. The assay limit of
detection depends on sample matrix, processing grade, DNA preparation and DNA content.