Description
Intended use:
SureFast® Hepatitis E PLUS is a real-time RT-PCR for the direct and qualitative detection of hepatitis E virus.
The test contains an Internal Control RNA (ICR, consisting of MS2-bacteriophage) as an internal control of sample preparation procedure and to determine possible PCR-inhibition.
General Information:
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm (FAM and VIC/HEX) at the same time. The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II, Roche Cobas z 480 Analyzer, Roche LightCycler® 2.0, Applied Biosystems 7500, LTF MyGo Pro and Qiagen RotorGene Q.
Accessories:
| Article Numbers | F7142 |
|---|---|
| Test format | 100 reactions |
| Sample preparation | For RNA-preparation the use of SureFast® PREP DNA/RNA Virus is recommended. |
| LOD (Detection limit) | The SureFast® Hepatitis E PLUS real-time RT-PCR has a limit of detection of 25 RNA copies. |
| Instructions |
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