SureFast® MRSA 4plex

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The SureFast® MRSA 4plex is a real-time PCR for the direct, qualitative detection and differentiation of methicillin-resistant Staphylococcus aureus (MRSA). The test detects the SCCmec/orfX junction, the mecA/mecC-gene and a specific DNA sequence of Staphylococcus aureus

 

Description

Intended use:

The SureFast® MRSA 4plex is a real-time PCR for the direct, qualitative detection and differentiation of methicillin-resistant Staphylococcus aureus (MRSA). The test detects the SCCmec/orfX junction, the mecA/mecC-gene and a specific DNA sequence of Staphylococcus aureus

 

General Information:

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500, Bio-Rad CFX96 and Agilent Mx3005P.

 

Accessories:

 

Specifications
Article Numbers F7117
Test format 100 reactions
Sample preparation SureFast® PREP Bacteria
LOD (Detection limit) The SureFast® MRSA 4plex real-time PCR has a limit of detection of < 5 DNA copies.
Files
Instructions

Additional information

Microbiological analysis