Description
Intended use:
The SureFast® STEC Screening PLUS is a real-time PCR for the direct, qualitative detection of DNA sequences of the virulence factors stx1 (subtype a-d) and stx2 (subtype a-g) of Escherichia coli (STEC).
Each reaction contains an internal amplification control (IAC).
General Information:
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche LightCycler® 2.0, Applied Biosystems 7500, Qiagen Rotor-Gene Q and R-Biopharm RIDA®CYCLER.
Accessories:
| Article Numbers | F5105 |
|---|---|
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation the use of SureFast® PREP Bacteria is recommended. |
| LOD (Detection limit) | The SureFast® STEC Screening PLUS real-time PCR has a limit of detection of ≤ 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content. |
| Instructions |
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