Description
Intended use:
The SureFast® Yersinia 3plex is a real-time PCR for the direct, qualitative detection and differentiation of specific ail gene DNA sequences of Yersinia pseudotuberculosis and Yersinia enterocolitica.
General Information:
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Applied Biosystems 7500, Qiagen Rotor-Gene Q and R-Biopharm RIDA®CYCLER.
Accessories:
| Article Numbers | F5132 |
|---|---|
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation the use of SureFast® PREP Bacteria is recommended. |
| LOD (Detection limit) | The SureFast® Yersina 3plex real-time PCR has a limit of detection of < 5 DNA copies. |
| Cross reactivity | The detection system of Yersinia pseudotuberculosis shows a cross reactivity to Yersinia pestis. |
| Instructions |
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