The SureFast® Yersinia 3plex is a real-time PCR for the direct, qualitative detection and differentiation of specific ail gene DNA sequences of Yersinia pseudotuberculosis and Yersinia enterocolitica.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Agilent AriaDx, Bio-Rad CFX96, Roche LightCycler® 480 II, Applied Biosystems 7500, Qiagen Rotor-Gene Q and R-Biopharm RIDA®CYCLER.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFast® PREP Bacteria is recommended.|
|LOD (Detection limit)||The SureFast® Yersina 3plex real-time PCR has a limit of detection of < 5 DNA copies.|
|Cross reactivity||The detection system of Yersinia pseudotuberculosis shows a cross reactivity to Yersinia pestis.|