The SureFood® ALLERGEN Insects is a real-time PCR for the direct, qualitative detection of specific insects (Insecta) DNA sequences.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Agilent AriaDx, Bio-Rad CFX96 Dx, Qiagen Rotor-Gene Q, R-Biopharm RIDA®CYCLER and Roche LightCycler® 480 II.
- SureFood® PREP Advanced
- SureFast® Mag PREP Food
- TANBead Maelstrom™ 8 Autostage
- TANBead Maelstrom™ 4800
|Test format||100 reactions|
|Sample preparation||For DNA preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.|
|LOD (Detection limit)||The SureFood® ALLERGEN Insects PCR has a limit of detection of ≤ 1 mg / kg using SureFood® PREP Advanced, protocol 1.|
|Cross reactivity||Cross reactivity was observed with DNA extracts from arachnids with 100 %.|