The SureFood® ALLERGEN Molluscs is a real-time PCR for the direct qualitative detection of specific molluscs (mollusca) DNA sequences according to directive (EC) 1169/2011.
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, AriaDx and Agilent Mx3005P.
|Test format||100 reactions|
|Sample preparation||For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.|
|LOD (Detection limit)||≤ 0.4 mg / kg|
|Evaluation||Real-time PCR instrument with two detection channels (510 nm and 580 nm)|